Artikel Ilmiah : A1D016141 a.n. NUR ATIN PURNAMASARI
| NIM | A1D016141 |
|---|---|
| Namamhs | NUR ATIN PURNAMASARI |
| Judul Artikel | ANALISIS MOLEKULER 16S rRNA BAKTERI RIZOSFER TANAMAN SINGKONG (Manihot esculenta) POTENSIAL PGPR (PLANT GROWTH PROMOTING RHIZOBACTERIA) DAN BIOASSAY PADA BEBERAPA VARIETAS PADI GOGO |
| Abstrak (Bhs. Indonesia) | Tujuan dari penelitian ini adalah: 1) menentukan identitas isolat bakteri TG-4 dan SR-2 hasil isolasi dari rizosfer tanaman singkong Desa Tanggeran dan Srowot, Banyumas melalui analisis bioinformatika sekuen 16S rRNA, 2) mengetahui hubungan kekerabatan dari kedua isolat dengan bakteri lain yang telah terdata di GenBank, 3) mengetahui kemampuan kedua isolat dalam menghasilkan hormon IAA (Indole Acetic Acid), dan 4) menentukan isolat bakteri terbaik terhadap pertumbuhan beberapa varietas padi lahan kering pada skala laboratorium. Penelitian dilaksanakan mulai Desember 2019 sampai Maret 2020 di Laboratorium Agroekologi dan Pemuliaan Tanaman Fakultas Pertanian, Universitas Jenderal Soedirman dan PT. Genetika Science Indonesia, Jakarta. Penelitian ini dilakukan dengan beberapa tahapan, yaitu identifikasi molekuler dengan BLAST-N, pengujian IAA secara kualitatif, dan bioassay. Pengujian isolat bakteri penghasil IAA dilakukan dengan mengkulturkan isolat ke dalam media NB (Nutrient Broth) yang disuplementasi L-triptofan 1000 ppm dan ditambah reagen Salkowski. Bioassay dilakukan dengan penanaman benih padi gogo varietas INPAGO UNSOED 1 (V1), INPAGO UNSOED Parimas (V2), dan INPAGO 8 (V3) di tanah ultisol setelah direndam oleh isolat bakteri sesuai perlakuan, yaitu B0 (kontrol), B1 (isolat TG-4), B2 (isolat SR-2), dan B3 (isolat konsorsium TG-4 & SR-2). Data dianalisis dengan uji F, dan setiap perbedaan nyata diuji lanjut dengan DMRT taraf kesalahan 5%. Hasil dari analisis BLAST-N menunjukkan bahwa isolat SR-2 memiliki homologi 100% dengan Bacillus paramycoides dan isolat TG4 memiliki homologi 98,77% dengan Bacillus albus. Bakteri TG-4 dan SR-2 terbukti mampu menghasilkan IAA. Hasil analisis bioassay menunjukkan bahwa tidak ada interaksi antara perlakuan varietas dengan pemberian isolat bakteri. Berdasarkan uji lanjut DMRT diperoleh bahwa aplikasi isolat konsorsium TG-4 & SR-2 (B3) memberikan pengaruh lebih tinggi terhadap pertumbuhan padi gogo. |
| Abtrak (Bhs. Inggris) | The objectives of this study were: 1) to identify TG-4 and SR-2 bacterial isolates previously isolated from the rhizosphere of cassava plants in Tanggeran and Srowot, Banyumas, through bioinformatics analysis of 16S rRNA sequence, 2) to determine the genetic kinship of TG-4 and SR-2 isolates with other bacteria that have been recorded in GenBank, 3) to determine the ability of TG-4 and SR-2 isolates in producing IAA (Indole Acetic Acid) hormone, and 4) to determine the best bacterial isolate for the growth of several upland rice varieties in Laboratory scale. The study was conducted from December 2019 to March 2020 in Agroecology and Plant Breeding Laboratory, Faculty of Agriculture, Jenderal Soedirman University and PT. Genetika Science Indonesia, Jakarta. This research was conducted in several stages, i.e. molecular identification with BLAST-N, qualitative IAA testing, and bioassay. Testing of IAA-producing bacterial isolates was carried out by culturing the isolates into NB (Nutrient Broth) media supplemented with 1000 ppm L-tryptophan and adding salkowski reagent. The bioassay was done by immersing upland rice seeds of INPAGO UNSOED 1 (V1), INPAGO UNSOED Parimas (V2), and INPAGO 8 (V3) with bacterial isolates according to the treatment which were B0 (control), B1 (TG-4 isolate), B2 (SR-2 isolate), and B3 (consortium isolates of TG-4 & SR-2). The seeds were then planted in a jar containing sterile Ultisol soil. Data were analyzed by F-test, and any significant differences were further analysed by DMRT with an error level of 5%. The results of BLAST-N analysis showed that SR-2 isolate exhibited 100% homology with Bacillus paramycoides and TG4 isolate revealed 98.77% homology with Bacillus albus. Both TG-4 and SR-2 bacterial isolates were confirmed to have the ability in producing IAA. The results of bioassay analysis showed that there was no interaction between the treatment of varieties with the application of bacterial isolate. Further DMRT determined that the application of consortium isolates of TG-4 & SR-2 (B3) gave a higher effect on the growth of upland rice. |
| Kata kunci | Gen 16S rRNA, BLAST-N, Plant growth promoting rhizobacteria (PGPR), Indole-3-acetic acid (IAA), padi gogo, dan tanah Ultisol. |
| Pembimbing 1 | Sapto Nugroho Hadi, S.Si., M. Biotech. |
| Pembimbing 2 | Ir. Imastini Dinuriah, M.Sc. |
| Pembimbing 3 | |
| Tahun | 2020 |
| Jumlah Halaman | 17 |
| Tgl. Entri | 2020-07-24 11:38:43.074393 |