Artikel Ilmiah : G1A015049 a.n. TALITHA APTA NITISARA

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NIMG1A015049
NamamhsTALITHA APTA NITISARA
Judul ArtikelPERBEDAAN MORFOLOGI SPERMATOZOA TIKUS PUTIH
(Rattus norvegicus) JANTAN PASCA INDUKSI BERBAGAI
MODEL STRES SLEEP DEPRIVATION
Abstrak (Bhs. Indonesia)ABSTRAK
Latar Belakang : Stres akibat paradoxical sleep deprivation (PSD) dan total sleep deprivation (TSD) dapat menyebabkan peningkatan stres oksidatif, mengaktivasi HPA, gangguan pada HPG yang berhubungan dengan infertilitas. Sleep recovery (SR) dapat menurunkan produksi radikal bebas dengan mekanisme pemulihan kadar antioksidan sehingga dapat memperbaiki fungsi reproduksi pria.
Tujuan : Mengetahui perbedaan morfologi spermatozoa pada tikus putih (Rattus norvegicus) jantan pasca induksi berbagai model stres sleep deprivation
Metode : Penelitian ini merupakan penelitian eksperimental dengan desain posttest only with control group. Tiga puluh ekor tikus putih dibagi secara acak menjadi 5 kelompok yakni 1 (kontrol sehat), 2 (PSD 20 jam/ hari selama 5 hari), 3 (TSD 24 jam/ hari selama 5 hari), PSD + SR (20 jam/ hari selama 5 hari dilanjutkan dengan sleep recovery selama 5 hari berikutnya), dan TSD + SR (24 jam/ hari selama 5 hari dilanjutkan dengan sleep recovery selama 5 hari berikutnya)
Hasil : Rerata persentase morfologi spermatozoa normal terendah terdapat pada kelompok 3 ( 13,00 ± 2,91), diikuti kelompok 5 (14,40 ± 2,70), kelompok 2 (18,00 ± 1,58), kelompok 4 (21,00 ± 1,58), dan kelompok tertinggi adalah kelompok 1 (22,60 ± 1,14). Uji One Way ANOVA menunjukkan terdapat perbedaan rerata yang signifikan (p<0,05). Uji Post-Hoc Tukey menunjukkan adanya perbedaan rerata yang signifikan (p<0,05) pada kelompok I-II, I-III, I-IV, I-V, II-III, III-IV, dan IV-V.
Kesimpulan : Terdapat perbedaan signifikan rerata persentase morfologi normal spermatozoa pada tikus putih (Rattus norvegicus) jantan pasca induksi berbagai model stres sleep deprivation
Kata kunci : Morfologi Spermatozoa, Paradoxical Sleep Deprivation, Sleep Recovery, Total Sleep Deprivation,
Abtrak (Bhs. Inggris)ABSTRACT
Background : Stress caused by paradoxical sleep deprivation (PSD) and total sleep deprivation (TSD) is able to increased oxidative stress, activated HPA, and disrupt of HPG which related with infertility. Sleep recovery (SR) can decreased free radical production by mechanism of recovery of antioxidant levels there by improving male reproductive function
Objective : To know the difference of spermatozoa morphology in male albino rats (Rattus norvegicus) after induction of various models of sleep deprivation stress
Method : This research was an experimental research with posttest only and control group design. Thirty male albino rats were distributed into 5 groups, group 1 (health control), group 2 (PSD 20 hours/day sleep deprivation for 5 days), group 3 (TSD 24 hours/day sleep deprivation for 5 days), group 4 (PSD + SR 20 hours/day sleep deprivation for 5 days continued with sleep recovery for the next 5 days), and group 5 (TSD + SR 24 hours/ day sleep deprivation for the first 5 days continued with sleep recovery for the next 5 days)
Result : The lowest mean of normal spermatozoa morphology percentage was in group 3 ( 13,00 ± 2,91), then group 5 (14,40 ± 2,70), group 2 (18,00 ± 1,58), group 4 (21,00 ± 1,58), and the highest was group 1 (22,60 ± 1,14). One way ANOVA showed sinificant differences (p<0,05). Post-Hoc Tukey showed significant differences (p<0,05) in group I-II, I-III, I-IV, I-V, II-III, III-IV, and IV-V.
Conclusion : There was significant difference of spermatozoa morphology male albino rats (Rattus norvegicus) after induction of various models of sleep deprivation stress.
Keywords : Paradoxical Sleep Deprivation, Sleep Recovery, Spermatozoa Morphology, Total Sleep Deprivation
Kata kunciMorfologi Spermatozoa, Paradoxical Sleep Deprivation, Sleep Recovery, Total Sleep Deprivation,
Pembimbing 1Dr. dr. Fitranto Arjadi, M. Kes
Pembimbing 2dr. Vitasari Indriani, M.M, M. Si. Med, Sp. PK
Pembimbing 3-
Tahun2019
Jumlah Halaman14
Tgl. Entri2019-02-20 06:19:54.175034
Cetak Bukti Unggah
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