Artikel Ilmiah : G1A015071 a.n. SONIA CAPIROSI AYUNINGTIAS
| NIM | G1A015071 |
|---|---|
| Namamhs | SONIA CAPIROSI AYUNINGTIAS |
| Judul Artikel | PERBEDAAN VIABILITAS SPERMATOZOA TIKUS PUTIH (Rattus norvegicus) JANTAN PASCA INDUKSI BERBAGAI MODEL STRES SLEEP DEPRIVATION |
| Abstrak (Bhs. Indonesia) | Latar Belakang : Stres yang disebabkan sleep deprivation dapat mempengaruhi regulasi hormon GnRH, LH, testosteron dan peningkatan level glukokortikoid yang menyebabkan penurunan viabilitas spermatozoa. Sleep recovery dapat memperbaiki interaksi aksis hypothalamic pituitary adrenal (HPA), penurunan peroksidase lipid dan radikal bebas, serta peningkatan kadar antioksidan Glutathione yang dapat memperbaiki dampak stres akibat sleep deprivation. Tujuan : Mengetahui perbedaan viabilitas spermatozoa pada tikus putih (Rattus norvegicus) jantan pasca induksi berbagai model stres sleep deprivation. Metode : Penelitian ini merupakan penelitian eksperimental dengan desain posttest only with control group. Tiga puluh ekor tikus putih dibagi secara acak menjadi 5 kelompok yaitu K I (kontrol sehat), K II (PSD selama 20 jam/hari selama 5 hari), K III (TSD 24 jam/hari selama 5 hari), K IV (PSD 20 jam/hari selama 5 hari pertama dan dilanjutkan dengan sleep recovery selama 5 hari berikutnya), dan K V (TSD 24 jam/hari selama 5 hari pertama dan dilanjutkan dengan sleep recovery selama 5 hari berikutnya). Hasil : Rerata viabilitas spermatozoa tertinggi terdapat pada K I (75,80 ± 4,56), diikuti K IV (67,60 ± 2,65), K II (66,90 ± 4,08), K V (33,70 ± 7,04), dan K III (31,00 ± 7,39). Uji One Way ANOVA menunjukkan terdapat perbedaan rerata yang signifikan (p<0,05) kemudian uji Post-Hoc Tukey menunjukkan adanya perbedaan rerata yang signifikan (p<0,05) pada kelompok I-III, I-V, II-III, II-V, III-IV dan IV-V. Kesimpulan : Terdapat perbedaan signifikan antara rerata viabilitas spermatozoa tikus putih (Rattus norvegicus) jantan pasca induksi berbagai model stres sleep deprivation. |
| Abtrak (Bhs. Inggris) | Background : Stress caused by sleep deprivation can affect the regulation of the GnRH, LH, testosterone and increase in glucocorticoid levels which causes a decrease in spermatozoa viability. Sleep recovery can improve the interaction of the hypothalamic pituitary adrenal (HPA) axis, decrease in lipid peroxidation and free radicals, and increase Glutathione antioxidant levels which can improve the impact of stress due to sleep deprivation. Objective : To know the difference of spermatozoa viability in male albino rats (Rattus norvegicus) after induction of various models of sleep deprivation stress. Method : This research was an experimental research with posttest only with control group design. Thirthy male albino rats were distributed into 5 groups, K I (health control), K II (PSD 20 hours/day sleep deprivation for 5 days), K III (TSD 24 hours/day sleep deprivation for 5 days), K IV (PSD 20 hours/day sleep deprivation for 5 days continued with sleep recovery for the next 5 days), K V (TSD 24 hours/day sleep deprivation for 5 days continued with sleep recovery for the next 5 days). Result : The highest mean of spermatozoa viability was in K I (75,80 ± 4,56), followed by K IV (67,60 ± 2,65), K II (66,90 ± 4,08), K V (33,70 ± 7,04), and K III (31,00 ± 7,39) . One way ANOVA showed sinificant differences (p<0,05) and Post-Hoc Tukey showed significant differences (p<0,05) in group I-III, I-V, II-III, II-V, III-IV and IV-V. Conclusion : There was significant difference of spermatozoa viability male albino rats (Rattus norvegicus) after induction of various models of sleep deprivation stress. |
| Kata kunci | Paradoxical Sleep Deprivation (PSD), Sleep Recovery (SR), Total Sleep Deprivation (TSD), Viabilitas Spermatozoa |
| Pembimbing 1 | Dr.dr.Fitranto Arjadi, M.Kes |
| Pembimbing 2 | dr.Vitasari Indriani, MM., Sp.PK, M.Si.Med |
| Pembimbing 3 | |
| Tahun | 2019 |
| Jumlah Halaman | 12 |
| Tgl. Entri | 2019-01-29 22:08:20.137998 |