| NIM | A1L014134 |
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| Namamhs | TERRA ASTIKA RAHMAWATI |
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| Judul Artikel | PENGARUH PEMBERIAN ZAT PENGATUR TUMBUH SPERMIDIN DAN 2,4-Dichlorophenoxyacetic Acid (2,4-D) TERHADAP PEMBENTUKAN KALUS ANTER JERUK PURUT (Citrus hystrix) PADA MEDIA MURASHIGE DAN SKOOG (MS) DAN CHU (N6) |
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| Abstrak (Bhs. Indonesia) | Salah satu perbaikan tanaman secara genetik dapat dilakukan menggunakan teknik kultur in vitro anter untuk menambah keragaman karakteristik tanaman jeruk. Penelitian kultur anter dilakukan untuk mengetahui pengaruh pemberian ZPT spermidin (SPD) dan asam 2,4-Diklorofenoksiasetat (2,4-D) pada media Murashige dan Skoog (MS) dan Chu (N6) serta konsentrasi SPD dan 2,4-D yang optimum pada kultur anter jeruk purut. Penelitian dilaksanakan mulai dari Desember 2017 sampai Oktober 2018 di Laboratorium Bioteknologi dan Pemuliaan Tanaman, Fakultas Pertanian, Universitas Jenderal Soedirman. Penelitian ini menggunakan Rancangan Petak Terbagi (Split Plot Design) dengan rancangan dasar Rancangan Acak Kelompok Lengkap (RAKL). Petak utama adalah perlakuan media yaitu media MS dan N6, sebagai anak petak yaitu perlakuan kombinasi spermidin dan 2,4-D. Konsentrasi spermidin yang digunakan yaitu 0 M, 10-4 M, dan 10-3 M, serta konsentrasi 2,4-D yang digunakan yaitu 0 mg/l, 1 mg/l, 2 mg/l. Kuncup bunga diberi praperlakuan cekaman dingin pada suhu 4°C selama 5 hari. Anter yang telah dikultur diinkubasi pada ruang kultur bersuhu 24°C sampai 8 minggu setelah tanam. Variabel yang diamati adalah waktu inisiasi kalus, jumlah inisiasi kalus, dan warna kalus atau warna eksplan. Data hasil penelitian dianalisis menggunakan Duncan Multiple Range Test (DMRT) pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa waktu rata-rata induksi tersingkat yaitu 13 hst pada konsentrasi 1 mg/l 2,4-D + 10-3 SPD. Formulasi media dan ZPT terbaik untuk menginduksi kalus sebesar 50,22 yaitu pada medium N6 dengan penambahan 1 mg/l 2,4-D. Warna eksplan pada minggu ke-8 setelah tanam yaitu kuning, kuning terang dan hijau kecoklatan. |
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| Abtrak (Bhs. Inggris) | One of plant genetic methods to improve was carried out to increase the diversity of characteristics of citrus plants, one of many genetic methods used is anther culture. This research aimed to determine the effect of growth regulator spermidine (SPD) and 2,4-Dicholophenoxyacetic acid (2,4-D) on Murashige and Skoog (MS) and Chu (N6) media and optimum concentration of SPD and 2,4-D for lime anther culture. The research was conducted from December 2017 to October 2018 at the Laboratory of Plant Breeding and Biotechnology, Faculty of Agriculture, Jenderal Soedirman University. The study used Split Plot Design with Randomized Complete Block Design (RCBD) basic design. The main plot is the media treatment, which were MS and N6 media, while the sub plot were: the combination treatment of SPD and 2,4-D. The concentration of SPD was 10-4 M, 10-3 M, 0 M and the concentration of 2,4-D was 0 mg/l, 1 mg/l, 2 mg/l. Flower buds were pre-treated with cold stress at 4 °C for five days. After anther culture procedure, anrher was incubated in a culture room with a temperature of 24 °C for eight weeks. The variables of his research were callus initiation time, callus initiation number, and callus color or explant color. Results of the research data were analyzed using Duncan Multiple Range Test (DMRT) at a significant level of 5%. The results showed that the shortest average induction time was 13 days at a concentration of 1 mg/l 2,4-D + 10-3 SPD. The best media formulation and the growth regulator to induce callus was N6 medium with the addition of 1 mg/l 2,4-D, that showed by number of callus 50,22. The color of explants at week after 8 weeks incubation were yellow, vivid yellow and olive brown. |
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| Kata kunci | kultur anter, media dasar MS dan N6, SPD, 2,4-D |
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| Pembimbing 1 | Prita Sari Dewi, S.P., M.Sc., Ph.D. |
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| Pembimbing 2 | Ir. Kharisun, Ph. D. |
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| Pembimbing 3 | |
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| Tahun | 2018 |
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| Jumlah Halaman | 14 |
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| Tgl. Entri | 2018-11-04 17:18:22.079416 |
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