Artikel Ilmiah : B1J013085 a.n. FATHIMAH AZ ZAHRO
| NIM | B1J013085 |
|---|---|
| Namamhs | FATHIMAH AZ ZAHRO |
| Judul Artikel | KEKERABATAN Streptomyces spp. YANG BERSIFAT ANTIBAKTERI BERDASARKAN GEN NONRIBOSOMAL PEPTIDE SYNTHETASE |
| Abstrak (Bhs. Indonesia) | Bakteri Streptomyces spp. asal rhizosfer tanaman mangrove Bruguiera sp., Sonneratia sp., Rhizophora sp., Aegiceras corniculatum dan Avicennia marina telah berhasil diisolasi. Bakteri Streptomyces spp. tersebut terbukti menghasilkan aktivitas antibakteri yang mampu menghambat pertumbuhan Stapylococcus aureus dan Eschericia coli. Seleksi isolat potensial dilakukan berdasarkan pada tingginya kemampuan isolat dalam memproduksi aktivitas antibakteri. Sebanyak lima isolat potensial dianalisis lebih lanjut pada tahap molekuler dan kekerabatan spesies. Penelitian ini bertujuan untuk mendeteksi adanya gen Nonribosomal Peptide Synthetase (NRPS) pada bakteri Streptomyces spp. yang mampu menghambat pertumbuhan S. aureus dan E. coli, mengetahui karakteristik gen tersebut dan mengetahui hubungan kekerabatan isolat Streptomyces spp. berdasarkan gen penyandi NRPS. Deteksi gen NRPS dilakukan melalui tahapan isolasi DNA bakteri Streptomyces potensial, amplifikasi gen NRPS melalui teknik PCR, elektroforesis gel, sekuensing DNA, analisis sekuen DNA menggunakan software BioEdit, analisis prediksi produk metabolit sekunder menggunakan program AntiSmash dan analisis filogenetik menggunakan program MEGA. Hasil analisis menunjukkan 5 isolat yang diteliti terdeteksi memiliki gen NRPS, dengan ukuran nukleotida sekitar 700 bp. Sebanyak 3 isolat berhasil terkualifikasi untuk dianalisis sekuensing. Berdasarkan jarak genetik pada sekuen setiap isolat, isolat Streptomyces sp. E401, Streptomyces sp. E403, dan Streptomyces sp. E461 memiliki nilai kekerabatan yang cukup dekat karena memiliki nilai jarak genetik 0,4-0,6. Hasil analisis sekuen homolog dapat digunakan untuk prediksi metabolit sekunder Streptomyces spp. Secara umum, prediksi sekuen NRPS Streptomyces spp. memiliki homologi dengan beberapa klaster gen, meliputi Streptomycin biosynthetic gene cluster dan Syringomycin biosynthetic gene cluster. Prediksi metabolit sekunder yang dihasilkan menunjukkan Streptomisin dan Siringomisin. Kata Kunci : Streptomyces, kekerabatan, gen NRPS, antibakteri, metabolit sekunder |
| Abtrak (Bhs. Inggris) | Streptomyces spp. bacteria obtained from. Bruguiera sp., Sonneratia sp., Rhizophore sp., Aegiceras corniculatum and Avicennia marina mangrove plants rhizosfer were isolated. Those Streptomyces spp. bacteria positively produced antibacterial activity that capable to inhibit the growth of Staphylococcus aureus and Escherichia coli. Selection of potential isolates was performed based on the high ability of isolates to produce antibacterial activity. Five potential isolates were further analyzed at the molecular and genetic relationship levels. This study aims to detect Non Ribosomal Peptide Synthetase (NRPS) encoding gene in Streptomyces spp. bacteria capable to inhibit the growth of S. aureus and E. coli, to know their gene characters and genetic relationship based on NRPS encoding gene. The research was performed by following steps: DNA isolation of potential Streptomyces spp. bacteria, Amplification of NRPS gene using PCR technique, Gel electrophoresys, DNA sequencing, Analysis of DNA sequence using BioEdit software, prediction analysis of secondary metabolite products using AntiSmash program and Phylogenetic analysis using MEGA program. The analysis showed that 5 isolates studied had NRPS encoding genes, with nucleotide size around 700 bps. Three isolates were successfully qualified for sequencing analysis. Based on genetic distance in each isolate sequence, Streptomyces sp. E401, Streptomyces sp. E403, and Streptomyces sp. E461 isolates showed high similarity because they had 0.4-0.6 genetic distance value. The homologous sequence analysis results can be used to predict secondary metabolites generated from Streptomyces spp. In general, predicted sequences have homology in several clusters of genes, including Streptomycin biosynthetic gene cluster and Syringomycin biosynthetic gene cluster. The secondary metabolites synthesized were predicted as Streptomycin and Siringomycin. Keywords: Streptomyces, genetic relationship, NRPS gene, antibacterial, secondary metabolites |
| Kata kunci | Streptomyces, genetic relationship, NRPS gene, antibacterial, secondary metabolites |
| Pembimbing 1 | Dr. Hendro Pramono, M.S |
| Pembimbing 2 | Dra. Dini Ryandini, M.Si |
| Pembimbing 3 | |
| Tahun | 2018 |
| Jumlah Halaman | 1 |
| Tgl. Entri | 2018-08-21 08:51:48.531872 |