| NIM | H1G014025 |
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| Namamhs | IFDA IYA SOLEHA |
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| Judul Artikel | Pengaruh Endosulfan (Pestisida) Terhadap Ekspresi Gen cGnRH-II Pada Ikan Nilem (Osteochilus hasseltii C.V.) Betina |
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| Abstrak (Bhs. Indonesia) | Endosulfan merupakan pestisida yang termasuk ke dalam golongan organoklorin dan banyak dipergunakan di Indonesia. Endosulfan yang masuk ke perairan akan terakumululasi pada biota perairan. Dampak yang ditimbulkan endosulfan yaitu mengganggu proses reproduksi melalui cGnRH-II yang berperan sebagai neuromodulator dan menstimulasi pelepasan hormon gonadotropin. Penelitian ini bertujuan untuk mengetahui konsentrasi dan lama waktu pemaparan endosulfan yang dapat mengganggu ekspresi gen cGnRH-II pada ikan nilem (Osteochilus hasseltii C.V.) betina. Metode yang digunakan adalah metode eksperimental Rancangan Acak Lengkap (RAL). Penelitian dilakukan dengan 4 tahap yaitu pengambilan hipotalamus, isolasi RNA, DNase Treatment, dan Real-Time PCR. Ikan nilem diberi 4 perlakuan selama 4 minggu dengan pengambilan sampel setiap 2 minggu sekali. Data kuantitatif berupa ekspresi gen penghasil cGnRH-II dianalisis dengan One-Way ANOVA. Hasil penelitian selama 4 minggu menunjukkan endosulfan belum memberikan pengaruh yang signifikan terhadap tingkat ekspresi gen cGnRH-II dengan nilai rata-rata ekspresi gen cGnRH-II berkisar antara 0,78 – 12,48. |
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| Abtrak (Bhs. Inggris) | Endosulfan is an organochlorine pesticide that widely used in Indonesia. Endosulfan that has entered in water column will accumulated in aquatic organisms. The impact of endosulfan for aquatic organisms is alter the reproduction process through cGnRH-II which play the role as neuromodulator and stimulate the gonadotropin hormones to release. The purpose of this study was to determine the concentration and exposure duration that can affect cGnRH-II gene expression in female Osteochilus hasseltii C.V. The method that used is experimental with Completely Randomize Design. The study was conducted with 4 stages, hypothalamus sampling, RNA isolation, DNase Treatment, and Real-Time PCR. O. hasseltii was given with four different treatments for four weeks, sampling was done for every two weeks. The quantitative data is in form as gene expression of cGnRH-II that has analyzed by One-Way ANOVA. The results of four weeks exposure showed that endosulfan has no significant effect to alter cGnRH-II gene expression with average range 0,78 – 12,48. |
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| Kata kunci | Endosulfan, Pemaparan, Ekspresi Gen, cGnRH-II. |
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| Pembimbing 1 | Dr. Norman Arie Prayogo, S. Pi., M. Si |
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| Pembimbing 2 | Drs. Asrul Sahri Siregar, M.Si |
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| Pembimbing 3 | |
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| Tahun | 2018 |
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| Jumlah Halaman | 8 |
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| Tgl. Entri | 2018-08-15 04:04:15.022979 |
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