| NIM | A1L014049 |
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| Namamhs | NANDA DELIA |
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| Judul Artikel | EKSPLORASI, IDENTIFIKASI DAN UJI BAKTERI ANTAGONIS Bacillus sp. DARI RIZOSFER JAGUNG TERHADAP BAKTERI LAYU STEWART |
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| Abstrak (Bhs. Indonesia) | Penelitian ini bertujuan untuk 1) mengeksplorasi dan identifikasi bakteri antagonis Bacillus sp. dari rizosfer jagung, 2) mengidentifikasi bakteri patogen layu Stewart pada tanaman jagung, 3) menguji efektivitas bakteri antagonis Bacillus sp. terhadap bakteri patogen secara in vitro, 4) mengetahui mekanisme penghambatan bakteri antagonis Bacillus sp. terhadap patogen layu Stewart. Penelitian dilaksanakan secara in vitro di Laboratorium Perlindungan Tanaman Fakultas Pertanian Universitas Jenderal Soedirman. Eksplorasi bakteri antagonis dan patogen layu Stewart diambil di pertanaman jagung daerah Grendeng. Penelitian ini dilaksanakan mulai bulan Oktober sampai Desember 2017. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap dengan 9 ulangan dan 3 perlakuan. Isolat bakteri yang diperoleh diidentifikasi menurut kunci identifikasi dan selanjutnya dilakukan uji postulat Koch. Variabel yang diamati adalah uji morfologi, uji biokimia seperti uji gram, uji katalase, uji oksidase, uji motilitas, uji hidrolisis pati, uji anaerobik, uji fisiologi pertumbuhan pada suhu 40˚C, uji patogenesitas dan uji in vitro. Hasil penelitian menunjukan bahwa Bacillus sp. ditemukan pada rizosfer jagung ditunjukan dengan ciri-ciri koloni bakteri circular (bulat) dan punctiform (bulat kecil), variasi margin dari entire (halus) dan lobate (berombak atau bergerigi), berwarna putih kusam, tidak berlendir, bakteri gram positif, berflagel dan sebagian bersifat motil (mampu bergerak), identifikasi patogen layu Stewart memiliki koloni berwarna kuning pada medium NA, kuning cerah pada medium Kings’B, memiliki keadaan koloni berlendir, non-motil, koloni berkilau, gram negatif, anaerob fakultatif, non-motil, katalase positif, oksidase negatif, hidrosis pati positif, uji pertumbuhan suhu 40˚C negatif, dan uji patogenisitas positif, Bacillus sp. yang paling efektif dalam menghambat bakteri patogen layu Stewart adalah Bacillus Bn1 dengan luas zona hambatan sebesar 3,29 mm2, mekanisme hambatan Bacillus sp. terhadap bakteri patogen layu Stewart bersifat bakteriostatik. |
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| Abtrak (Bhs. Inggris) | This research aimed to: (1) explore and identify antagonist bacterial of Bacillus sp. from maize rizospher, (2) identify of pathogenic bacteria stewart wilt in maize crop, (3) test the effectivennes antagonist bacterial of Bacillus sp. against pathogenic bacterial by in vitro; (4) know the mechanism of inhibition antagonist bacterial of Bacillus sp. against stewart’s wilt. The research was carried out by in vitro at the Laboratory of Plant Protection, Faculty of Agriculture, Jenderal Soedirman University. Exploration of antagonistic bacteria and Stewart wilter pathogens was taken at the Grendeng maize crop. The research was conducted from October to December 2017. The research completely randomized design was used with 9 replicates and 3 treatments. The bacterial isolates obtained were identified by key identification and then Koch postulates were tested. The variables observed were morphological test, biochemical test such as gram test, catalase test, oxidase test, motility test, starch hydrolysis test, anaerobic test, growth physiology test at 40˚C, pathogenicity test and in vitro test. Results of the research showed that Bacillus sp. found in the corn rhizosphere is characterized by circular (bulbous) and punctiform (rounded) bacterial colonies, variations in margins of the entire (fine) and lobate (wavy or jagged), dull white, not slimy, gram-positive bacteria, staggered and motionally immobilized, identification of Stewart wilt pathogens having yellow colonies on NA medium, bright yellow in Kings'B medium, having slimy, non-motile, sparkling, gram negative, facultative anaerobic, -motil, positive catalase, negative oxidase, positive starch hydrosis, 40˚C negative temperature growth test, and positive pathogenicity test, Bacillus sp. the most effective in inhibiting Stewart's wilter pathogen bacteria was Bacillus Bn1 with an area of 3.29 mm 2 inhibition zone, the barrier mechanism of Bacillus sp. against Stewart wilt pathogen bacteria is bacteriostatic. |
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| Kata kunci | identifikasi, Bacillus sp., layu Stewart, jagung. |
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| Pembimbing 1 | Dr.ir. Heru Adi Djatmiko, M.P |
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| Pembimbing 2 | Dr.Ir. Nur Prihatiningsih, M.S |
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| Pembimbing 3 | |
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| Tahun | 2018 |
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| Jumlah Halaman | 16 |
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| Tgl. Entri | 2018-04-13 08:48:46.635157 |
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