Artikel Ilmiah : B1J012152 a.n. AN NISAA' JUSTICIA MUTIARA RANY
| NIM | B1J012152 |
|---|---|
| Namamhs | AN NISAA' JUSTICIA MUTIARA RANY |
| Judul Artikel | PERAN BAKTERI RIZOSFER PENGHASIL ACC DEAMINASE PADA PERKECAMBAHAN DAN PERTUMBUHAN VEGETATIF TANAMAN KEDELAI (Glycine max) DI TANAH SALIN |
| Abstrak (Bhs. Indonesia) | Lahan salin memiliki kandungan ion Na+ dan Cl- yang tinggi. Kandungan kedua ion tersebut dapat bersifat toksik bagi tanaman dan menyebabkan produksi hormon etilen meningkat. Peningkatan konsentrasi hormon etilen dapat menghambat pertumbuhan dan perkembangan perakaran. Enzim ACC (1-Aminocyclopropane-1-carboxylic acid) deaminase diketahui mampu mengendalikan biosintesis hormon etilen yang diproduksi secara berlebih. Bakteri rizosfer adalah salah satu kelompok bakteri yang mampu menghasilkan enzim ACC deaminase. Penelitian ini bertujuan untuk mengetahui kemampuan isolat bakteri rizosfer dalam menghasilkan enzim ACC deaminase, mengetahui pengaruh lingkungan (suhu, salinitas, pH dan herbisida) terhadap kemampuan isolat bakteri rizosfer dalam menghasilkan ACC deaminase dan peran isolat bakteri rizosfer penghasil ACC deaminase dalam mendukung perkecambahan dan pertumbuhan vegetatif tanaman kedelai (Glycine max) di tanah salin. Penelitian tentang kemampuan isolat bakteri rizosfer dalam menghasilkan enzim ACC deaminase dan pengaruh lingkungan (temperatur, salinitas, pH dan herbisida) terhadap kemampuan isolat bakteri rizosfer dalam menghasilkan ACC deaminase dilakukan secara deskriptif. Penelitian secara eksperimental dengan Rancangan Acak Lengkap dilaukan untuk mengetahui peran isolat bakteri rizosfer pada perkecambahan dan pertumbuhan vegetatif tanaman kedelai (Glycine max) di tanah salin. Medium DF (Dworkin Foster) dengan penambahan ACC sebagai substrat digunakan untuk menguji kemampuan isolat bakteri rizosfer dalam menghasilkan ACC deaminase secara kualitatif. Uji ninhidrin dilakukan untuk mengukur kadar ACC sisa pemecahan ACC deaminase. Pengaruh isolat bakteri rizosfer penghasil ACC deaminase terhadap tanaman kedelai (Glycine max) dilihat dari kemampuan perkecambahan dan pertumbuhan vegetatif tanaman kedelai (Glycine max) pada media pasir pantai. Data parameter pertumbuhan tanaman kedelai (Glycine max) dianalisis menggunakan Analisis Ragam (ANOVA) pada tingkat kepercayaan 95 %. Isolat bakteri dilakukan karakterisasi kemampuan fisiologis sebagai kandidat PGPR. Hasil penelitian menunjukkan bahwa beberapa isolat bakteri asal rizosfer mampu menghasilkan enzim ACC deaminase. Pengaruh lingkungan (temperatur, salinitas, pH dan herbisida) berpengaruh terhadap kemampuan isolat bakteri dalam menghasilkan ACC deaminase. Isolat bakteri rizosfer penghasil ACC deaminase berpengaruh terhadap bobot basah, bobot kering dan tinggi tajuk tanaman kedelai namun tidak berpengaruh terhadap panjang akar tanaman. Isolat bakteri uji asal rizosfer memiliki karakter fisiologis sebagai kandidat PGPR dengan kemampuan penambatan N2, menghasilkan IAA, melarutkan fosfat, menghasilkan enzim protease dan selulase. |
| Abtrak (Bhs. Inggris) | Saline fields have a high content of Na+ and Cl- ions. The content of these two ions can be toxic to plants and cause the production of ethylene hormones to increase. Increased concentrations of ethylene may inhibit the growth and development of rooting. The enzyme ACC (1-Aminocyclopropane-1-carboxylic acid) deaminase is known to control the biosynthesis of excessively produced ethylene hormones. Rizosphere bacteria is one of the groups of bacteria capable of producing enzyme ACC deaminase. This study aims to determine the ability of bacteria rhizosphere in generating enzyme ACC deaminase, determine the effect of the environment (temperature, salinity, pH and herbicides) on the ability of bacteria rhizosphere in producing ACC deaminase and the role of bacteria rhizosphere producing ACC deaminase in favor germination and vegetative growth Soybean plant (Glycine max) in saline soil. Research on the ability of bacteria in the rhizosphere produce ACC deaminase enzyme and the influence of the environment (temperature, salinity, pH and herbicides) on the ability of bacteria to produce ACC deaminase rhizosphere descriptively. Experimental research to take place completely randomized design to determine the role of bacteria in the rhizosphere of germination and vegetative growth of soybean (Glycine max) in saline soil. Medium DF (Dworkin Foster) with the addition of ACC as a substrate was used to test the ability of rhizosphere bacterial isolates to produce ACC deaminase qualitatively. The ninhydrin test was performed to measure ACC levels of residual breakage of ACC deaminase. Effect of rhizosphere bacterial isolates producing ACC deaminase on soybean (Glycine max) seen from the ability of germination and vegetative growth of soybean (Glycine max) on beach sand media. The data of soybean plant growth parameter (Glycine max) was analyzed using Analysis of Variety (ANOVA) at 95% confidence level. Bacterial isolates performed characterization of physiological abilities as PGPR candidates. The results showed that some bacterial isolates from rhizosphere were able to produce ACC deaminase enzyme. The influence of the environment (temperature, salinity, pH and herbicide) influences the ability of bacterial isolates to produce ACC deaminase. Isolate of ACC deaminase producing rhizosphere bacteria had an effect on wet weight, dry weight and height of canopy of soybean crop but did not affect the root length of the plant. Rhizosphere bacterial isolates original test has physiological characteristics as a candidate PGPR with tethering capability N2, produce IAA, dissolving phosphate, produces an enzyme protease and cellulase. |
| Kata kunci | Bakteri rizosfer, ACC deaminase, kedelai, salinitas. |
| Pembimbing 1 | Dr. Oedjijono, M.Sc. |
| Pembimbing 2 | Dr. rer. nat. Sarjiya Antonius |
| Pembimbing 3 | |
| Tahun | 2017 |
| Jumlah Halaman | 10 |
| Tgl. Entri | 2017-08-14 21:10:13.477729 |