| NIM | A1L013005 |
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| Namamhs | TRISMA NUR JANAH |
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| Judul Artikel | KULTUR IN VITRO JERUK KEPROK (Citrus nobilis) MENGGUNAKAN EKSPLAN RUAS KOTILEDON DENGAN PERLAKUAN BAP. |
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| Abstrak (Bhs. Indonesia) | Tujuan dari penelitian ini adalah 1) mengetahui pengaruh konsentrasi BAP 0 mg/l, 1 mg/l dan 3 mg/l pada kultur in vitro ruas kotiledon jeruk keprok, 2) menentukan konsentrasi BAP terbaik terhadap kultur in vitro ruas kotiledon jeruk keprok, dan 3) mengetahui respon eksplan ruas kotiledon pada kultur in vitro jeruk keprok. Penelitian dilaksanakan di Laboratorium Pemuliaan Tanaman dan Bioteknologi Fakultas Pertanian Universitas Jenderal Soedirman Purwokerto mulai November 2016 sampai Maret 2017. Penelitian ini menggunakan Rancangan Acak Kelompok Lengkap (RAKL) dengan tiga perlakuan, dan tiga ulangan. Perlakuan meliputi BAP 0 mg/l, 1 mg/l, dan 3 mg/l. Variabel yang diamati meliputi perubahan warna kalus, saat muncul tunas pertama, jumlah tunas, tinggi tunas, jumlah daun, dan saat muncul kalus. Hasil penelitian menunjukkan bahwa kultur in vitro dengan menggunakan ruas kotiledon pada konsentrasi BAP 0 mg/l, 1 mg/l dan 3 mg/l dapat meningkatkan jumlah tunas, jumlah daun, dan tinggi tunas. Perlakuan konsentrasi 1 mg/l memberikan hasil terbaik dalam meningkatkan jumlah tunas sebanyak 2, 61 tunas, tinggi tunas sebesar 0, 77 cm, dan jumlah daun sebanyak 6,42 helai. Penggunaan eksplan ruas kotiledon memberikan respon yang baik dalam menginduksi tunas. Ditandai dengan munculnya 42 tunas adventif dan 28 tunas aksilar |
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| Abtrak (Bhs. Inggris) | This research aimed to 1) study the effect of BAP with concentration 0 mg/l, 1 mg/l and 3 mg/l on in vitro culture in keprok orange cotyledon segments, 2) to determine which BAP concentration that has the best effect on in vitro culture in keprok orange cotyledon segments, and 3) to study explants response of cotyledon segments toward in vitro culture efforts of keprok orange. This research was conducted in the Laboratory of Plant Breeding and Biotechnology Faculty of Agriculture, the Jenderal Soedirman University, from November 2016 to March 2017. The experimental design used in this research was Completely Randomized Block Design (CRBD) with three treatment and three times replications. The treatments were consisted of BAP 0 mg/l, 1 mg/l, and 3 mg/l. Observed variables were discoloration of callus, time of first emerging buds, the number of buds, the length of buds, the number of leaves, and the time of the first emerging callus. The results of this research showed that the in vitro culture using cotyledon segments on BAP concentration of 0 mg/l, 1 mg / l and 3 mg/l could induce buds number, leaves number, and buds height. The results of this research showed that the in vitro culture using cotyledon segments on BAP concentration of 0 mg l, 1 mg/l and 3 mg/l could induce buds number, leaves number, and buds height. Concentration 1 mg/l showed the best result in improving the number of buds as 2,63 buds, the length of bud as 0,77 cm, and the number of leaves as 6,42 leaf. The use of cotyledon segment explant has gave good response in induces buds growth. Showed by the emerging of 42 adventitious buds and 28 axillary buds. |
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| Kata kunci | jeruk keprok, ruas kotiledon, dan BAP. |
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| Pembimbing 1 | Prita Sari Dewi, S.P., M.Sc., Ph.D. |
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| Pembimbing 2 | Siti Nurchasanah, S.P., M.Si. |
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| Pembimbing 3 | |
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| Tahun | 2017 |
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| Jumlah Halaman | 9 |
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| Tgl. Entri | 2017-08-06 17:56:31.393082 |
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