| Abstrak (Bhs. Indonesia) | Penelitian bertujuan untuk mengkaji pengaruh waktu post thawing semen beku kambing boer terhadap motilitas, dan integritas membran spermatozoa. Penelitian dilaksanakan di Laboratorium Fisiologi dan Reproduksi Ternak Terapan, Fakultas Peternakan, Universitas Jenderal Soedirman, Purwokerto pada tanggal 13-17 Februari 2017. Metode yang digunakan adalah metode eksperimen dengan menggunakan Rancangan Acak Lengkap (RAL). Perlakuan yang diuji adalah waktu post thawing (thawing pada suhu 37°C selama 30 detik) semen beku yang terdiri atas P0 (0 menit), P1 (30 menit), P2 (60 menit), dan P3 (90 menit). Setiap perlakuan diulang sebanyak enam kali. Peubah yang diukur adalah Motilitas dan Integritas Membran Spermatozoa. Data dianalisis menggunakan analisis variansi dan dilanjutkan dengan uji orthogonal polynomial dan uji beda nyata jujur. Hasil analisis menunjukan waktu post thawing memberikan pengaruh linear yang sangat nyata (P<0,01) dan perbandingan yang nyata (P<0,05) terhadap motilitas dan integritas membran spermatozoa. Rataan motilitas dan integritas membran sama-sama mengalami penurunan seiring dengan semakin lama waktu post thawing yaitu P0 (47,17 ± 0,76 %); P1 (43,00 ± 0,91 %); P2 (38,16 ± 1,48 %); P3 (31,41 ± 0,55 %) dan P0 (54,98 ± 1,00 %); P1 (46,70 ± 1,07 %); P2 (40,07 ± 0,96 %); P3 (34,12 ± 1,05 %). Kesimpulan, semakin lama waktu post thawing dapat menurunkan motilitas dan integritas membran spermatozoa. Waktu post thawing semen beku Kambing Boer lebih dari 30 menit kurang layak untuk dilakukan inseminasi buatan. |
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| Abtrak (Bhs. Inggris) | This research aimed to know the effect of post thawing time of boer frozen semen on motility, and integrity membrane of spermatozoa. This research was conducted at The Laboratory of Animal Physiology and Applied Reproduction, Faculty of Animal Science, Jenderal Soedirman University, Purwokerto from 13 th - 17 th February, 2017. The research was conducted experimentaly using the Completely Randomized Design. The treatments tested were post thawing time (thawing at temperature of 37°C for 30 seconds) frozen semen consisting of P0 (0 minute); P1 (30 minutes); P2 (60 minutes); P3 (90 minutes). Each treatment was repeated six times. The parameters measured were motility and intact plasma membrane. Data were analyzed using analysis of variance followed by polynomial orthogonal test and honestly significant difference test. The analysis result showed that post thawing time gave very significant linear effect (P <0.01) and significant comparison (P <0.05) on motility and productivity of spermatozoa membrane. The mean of motility and integrity membrane decreased in line with the length of post thawing time were P0 (47.17 ± 0.76 %); P1 (43.00 ± 0.91 %); P2 (38.16 ± 1.48 %); P3 (31.41 ± 0.55 %) and P0 (54.98 ± 1.00 %); P1 (46.70 ± 1.07 %); P2 (40.07 ± 0.96 %); P3 (34.12 ± 1.05 %), respectively. The conclusion, the longer of post thawing time can decrease motility and integrity membrane of spermatozoa. Post thawing time of Boer frozen semen over 30 minutes is less feasible for artificial insemination. |
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