Artikel Ilmiah : A1L013052 a.n. FITRIANTI
| NIM | A1L013052 |
|---|---|
| Namamhs | FITRIANTI |
| Judul Artikel | KAJIAN APLIKASI METABOLIT SEKUNDER TIGA ISOLAT Pseudomonas fluorescens TERHADAP PENYAKIT ANTRAKNOSA DAUN KAKAO |
| Abstrak (Bhs. Indonesia) | Penelitian bertujuan mengkaji keefektifan metabolit sekunderisolat tiga isolat Pseudomonas fluorescens yaitu isolat P60,P20, dan P8 terhadap penyakit antraknosa daun kakao,serta pengaruhnya terhadap pertumbuhan tanaman kakao. Penelitian dilaksanakan di perkebunan kakao rakyat, Dusun Plumbungan, Desa Putat, Kecamatan Patuk, Kabupaten Gunung Kidul, Yogyakarta, mulai November 2016 sampai Januari 2017. Penelitian menggunakan Rancangan Acak Kelompok dengan 4 ulangan dan 7 perlakuan terdiri atas kontrol, aplikasi metabolit sekunder P. fluorescens P60,P. fluorescens P20, P. fluorescens P8, kombinasiP. fluorescens P60+P20, P. fluorescens P60+P8 dan P. fluorescens P20+P8. Variabel yang diamati meliputi intensitas penyakit, laju infeksi, jumlah tunas sehat, dan kandungan senyawa fenol.Hasil penelitian menunjukkan bahwa perlakuanmetabolit sekunder P. fluorescens P60, P20dan P8 secara tunggal dan gabungan mampu menekan intensitas penyakit berkisar 42,01-54,50%. Laju infeksi perlakuan metabolit sekunder P. fluorescens P60,P. fluorescens P20, P. fluorescens P8, kombinasiP. fluorescens P60+P20, P. fluorescens P60+P8 dan P. fluorescens P20+P8 berturut-turut 0,23; 0,25; 0,26; 0,26; 0,31; dan 0,24 unit/hari. Metabolit sekunderP. fluorescens P60 mampu meningkatkan jumlah tunas sehat sebesar 67,44%. Perlakuan P. fluorescens P60 mampu meningkatkan senyawa fenol (saponin, tanin dan glikosida) pada daun kakao. |
| Abtrak (Bhs. Inggris) | This research aimed to study the effectiveness of secondary metabolites derived from three Pseudomonas fluorescens isolates towardcocoaleaves anthracnose, and their influence on the growth of cocoa plants. The research was conducted at the cocoa plantations at Plumbungan hamlet, Putat Village, Patuk Sub-District, Gunung Kidul Regency, Yogyakarta from November 2016 up to January 2017. Randomized block design was used with four replicates andseven treatments consisted of control, application of secondary metabolite from P. fluorescens P60, P. fluorescens P20, P. fluorescens P8, combination between P. fluorescens P60 + P20, P. fluorescens P60 + P8 and P. fluorescens P20 + P8. Variable observed were the disease intensity, the infection rate, the number of healthy shoots andphenoliccompound. Result of the research showed that the secondary metabolite fromP. fluorescens P60, P20 and P8 aloneor in combination could suppress the diseaseintensity, in between 42.01-54.50%. The infection rate cause by metabolite secondary of P. fluorescens P60, P. fluorescensP20,P8, P. fluorescens, combination between P. fluorescens P60+P20, P. fluorescensP60+P8 and P. fluorescens P20+P8 as 0.23; 0.25; 0.26; 0.26; 0.31; and 0.24 units/day respectively.The secondary metabolite ofP. fluorescens P60 could increas the number of healthy shoots as 67.44 %.The secondary metabolite ofP. fluorescens P60 could increas phenolic compound (tannin,saponin and glycosides) in cocoa leaves. |
| Kata kunci | Kakao, Metabolit Sekunder, Pseudomonas fluorescens, antraknosa daun. |
| Pembimbing 1 | Prof. Ir. Loekas Soesanto, M.S., Ph.D |
| Pembimbing 2 | Ir. Abdul Manan, M.P |
| Pembimbing 3 | |
| Tahun | 2017 |
| Jumlah Halaman | 10 |
| Tgl. Entri | 2017-07-24 17:36:21.947462 |