| NIM | A1L013093 |
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| Namamhs | KURNIASIH MUCHAROMAH |
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| Judul Artikel | Isolasi, Identifikasi, dan Uji Virulensi Penyakit Noda Hitam Kelabu pada Buah Pepaya California |
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| Abstrak (Bhs. Indonesia) | Penelitian ini bertujuan untuk mengisolasi, mengidentifikasi penyebab penyakit, dan menguji tingkat virulensi penyebab penyakit noda hitam kelabu pada pepaya California. Penelitian dilaksanakan pada Agustus 2016 sampai November 2016 di Laboratorium Perlindungan Tanaman Fakultas Pertanian, Universitas Jenderal Soedirman, dengan pengambilan sampel pepaya sakit di Desa Cibangkong, Kecamatan Pekuncen, Kabupaten Banyumas dan pepaya sehat di Desa Pliken, Kecamatan Sokaraja, Kabupaten Banyumas. Identifikasi dilaksanakan dengan metode deskriptif, sedangkan pengujian in vivo menggunakan Rancangan Acak Lengkap dengan dua faktor dan lima ulangan. Faktor letak buah berdasarkan letak vertikal buah pada batang, yaitu T1 (buah pada letak vertikal pertama), T2, T3, T4, dan T5. Faktor inokulasi terdiri atas 1 (diinokulasi patogen) dan K (tanpa diinokulasi patogen). Setiap unit percobaan terdiri atas 3 buah pepaya dengan 2 pepaya diinokulasi dan satu pepaya tanpa inokulasi (kontrol). Variabel pengamatan yaitu gejala dan tanda penyakit, makroskopis dan mikroskopis patogen, kekerasan buah, masa inkubasi, luas serangan, dan intensitas penyakit. Hasil penelitian menunjukkan bahwa penyakit pada sampel buah pepaya dengan gejala bercak kecil berwarna kekuningan hingga coklat kehitaman adalah penyakit noda hitam kelabu, penyebabnya jamur Corynespora cassiicola (Berk. & Curt.) Wei. C. cassiicola mempunyai koloni berwarna coklat kehitaman, permukaan koloni halus seperti kapas, bentuk permukaan melengkung, tepi koloni rata, warna balik koloni abu-abu sampai hitam. Jamur berwarna putih, kemudian berubah semakin gelap sesuai umur. C. cassiicola mempunyai konidiofor tegak, sedikit bengkok, bersepta, tunggal dan ada yang bercabang, warna konidiofor coklat, konidium ada yang tunggal dan bercabang, sedikit bengkok, dan bersepta 2-12. C. cassiicola mempunyai virulensi sedang. Masa inkubasi tercepat pada T4-1 sebesar 3,40 hsi, luas serangan tertinggi pada T1-1 sebesar 494,4 mm2, dan intensitas penyakit tertinggi sebesar 30,67% pada T1-1. |
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| Abtrak (Bhs. Inggris) | This research aimed to isolate, identify causal agent of the disease, and test virulent level of the disease causing black-gray stain on Californian papaya. This research has been done in August until November 2016 at the Plant Protection Laboratory of Agriculture Faculty, Universitas Jenderal Soedirman, by taking sample of diseased Californian papaya at Penjalin hamlet of Cibangkong Village, Pekuncen Subdistrict, Banyumas Regency and of healthy one at Pliken Village, Sokaraja Subdistrict, Banyumas Regency. Identification was done with descriptive methods, while the in vivo test used randomized complete design with two factors and repeated five times. Fruit layout factors based on the layout of vertical stem and fruit on the same tree consisted of T1 (fruit on the first layout), T2, T3, T4 to T5 (fruit on the fifth one). Factor inoculation consisted of inoculated pathogen (1) and no inoculated pathogen (K). Each experimental unit consisted of 3 papayas arranged by two inoculated papayas and one without inoculation (control). The observed variables included symptoms and signs of the disease, macroscopic and microscopic pathogen morphology, fruit hardness, incubation period, the attack area and the intensity of the disease. The results showed that the disease found in the papaya samples with the symptoms of small patches of yellowish to dark brown was black gray stain disease. The disease was caused by fungus Corynespora cassiicola (Berk. & Curt.) Wei. C. cassiicola had blackish brown colony, the colony's smooth surface textures such as cotton, form a curved surface, the color behind the colony of gray to black and the edges of the colony. Originally white mushrooms, then changed the darker the appropriate age. C. cassiicola had conidiophore upright, slightly bent, septa, single and some were branched, brown color conidiophore, conidia with a single and branched out, slightly bent, and 2-12 septa. Virulence test results showed that C cassiicola had high virulence indicated by the incubation period and attack area that was not affected by the layout of the fruit. The fastest incubation period occurred on fruit on the layout of vertical fourth inoculated pathogen, i.e., 3,40 dai (day after inoculation), the largest attack area occurred in fruit on the first vertical layout of the inoculated pathogen, i.e., 494,4 mm2 and the intensity of the disease i.e., 30,67% on the first vertical layout of the inoculated pathogen. |
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| Kata kunci | Isolasi, Identifikasi, Uji Virulensi, Penyakit Noda Hitam Kelabu, Pepaya California. |
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| Pembimbing 1 | Prof. Ir. Loekas Soesanto, M.S., Ph.D |
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| Pembimbing 2 | Prof. Dr. Ir. Suwarto, M.S. |
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| Pembimbing 3 | |
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| Tahun | 2017 |
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| Jumlah Halaman | 16 |
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| Tgl. Entri | 2017-03-31 17:29:17.266254 |
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