| NIM | A1L012185 |
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| Namamhs | YUNIAR WULANSARI |
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| Judul Artikel | APLIKASI METABOLIT SEKUNDER DUA ISOLAT Pseudomonas fluorescens SECARA TUNGGAL DAN GABUNGAN UNTUK MENGENDALIKAN PENYAKIT ANTRAKNOSA PADA PISANG MAS LEPAS PANEN |
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| Abstrak (Bhs. Indonesia) | Penelitian ini bertujuan untuk mengetahui pengaruh metabolit sekunder dua isolat P. fluorescens dalam menghambat pertumbuhan Colletotrichum musae in vitro, dalam mengendalikan penyakit antraknosa in vivo, dan mengetahui pengaruhnya terhadap mutu buah pisang lepas panen. Pada uji in vitro digunakan Rancangan Acak Lengkap, sedangkan pada uji in vivo menggunakan Rancangan Acak Kelompok, masing-masing diulang sebanyak enam kali. Perlakuan terdiri atas kontrol, metabolit sekunder P. fluorescens P60, metabolit sekunder P. fluorescens P32, serta gabungan P. fluorescens P60+P. fluorescens P32. Variabel yang diamati yaitu daya hambat, masa inkubasi, intensitas penyakit, luas serangan, tingkat kekerasan, kadar gula, serta uji organoleptik. Hasil penelitian menunjukkan bahwa perlakuan metabolit sekunder P. fluorescens P60 mampu menghambat pertumbuhan C. musae sebesar 54%, dan perlakuan gabungan metabolit sekunder P. fluorescens P60+ P. fluorescens P32 sebesar 56%. Pada uji in-vivo perlakuan gabungan metabolit sekunder P. fluorescens P60+ P. fluorescens P32 mampu menekan intensitas penyakit antraknosa sebesar 33,44%, dan metabolit sekunder P. fluorescens P60 secara tunggal sebesar 33,39%. Semua perlakuan metabolit sekunder P. fluorescens mampu menekan luas serangan (metabolit sekunder P. fluorescens P60 sebesar dan 26,15%, metabolit sekunder P. fluorescens P32 sebesar 14,20%, dan gabungan metabolit sekunder P. fluorescens P60+ P. fluorescens P32 sebesar 28,32%). Perlakuan metabolit sekunder P. fluorescens P60 dan gabungan metabolit sekunder P. fluorescens P60+P. fluorescens P32 memengaruhi mutu pada kadar gula dan warna buah pisang, namun semua perlakuan tidak memengaruhi kekerasan dan faktor organoleptis. |
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| Abtrak (Bhs. Inggris) | This research aimed to know secondary metabolites effect of two Pseudomonas fluorescens isolates in inhibiting Colletotrichum musae growth in vitro, in controlling anthracnose in vivo, and on quality of postharvest banana. Completely randomized Design was used in in vitro test, while Randomized Block Design was used in in vivo test, each repeated six times. The treatments were control, secondary metabolite of P. fluorescens P60, secondary metabolite of P. fluorescens P32, and the secondary metabolite combination of P. fluorescens P60+P. fluorescens P32. Variables observed were inhibition zone, incubation periode, inhibition level, disease intensity, attack area, soften level, sugar content, and organoleptic test. The research result indicated that secondary metabolite of P. fluorescens P60 and the combination were able to inhibit the pathogen growth in vitro as 54 and 56%, respectively. In in vivo test, the secondary metabolite of P. fluorescens P60 and the combination could inhibit disease intensity as 34.44 and 46.06%, respectively. All secondary metabolites of P. fluorescens could inhibit attact area as 28.3, 26.15, and 14,20%, respectively and increase fruit quality in sugar content and colour of banana fruit, but all treatments did not influence harderness and organoleptic factor. |
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| Kata kunci | : Antraknosa, pisang lepas panen, metabolit sekunder, Pseudomonas fluorescens |
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| Pembimbing 1 | Prof. Ir. Loekas Soesanto, M.S., Ph.D. |
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| Pembimbing 2 | Ir. Abdul Manan, M.P. |
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| Pembimbing 3 | |
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| Tahun | 2016 |
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| Jumlah Halaman | 10 |
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| Tgl. Entri | 2016-07-20 08:52:58.394632 |
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