Artikel Ilmiah : A1L012003 a.n. LUSI YULANDARI

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NIMA1L012003
NamamhsLUSI YULANDARI
Judul ArtikelUJI APLIKASI METABOLIT SEKUNDER DUA ISOLAT Trichoderma sp. TERHADAP PENYAKIT PEMBULUH KAYU
(Oncobasidium theobromae Talbot & Keane)
PADA BIBIT KAKAO
Abstrak (Bhs. Indonesia)Penelitian ini bertujuan untuk mengkaji keefektifan aplikasi metabolit sekunder Trichoderma sp., isolat jahe dan bawang merah, maupun gabungannya, terhadap penyakit pembuluh kayu dan pengaruhnya terhadap pertumbuhan serta kandungan senyawa fenol pada bibit kakao. Penelitian telah dilaksanakan di Laboratorium Perlindungan Tanaman, Fakultas Pertanian, Universitas Jenderal Soedirman, Purwokerto dan Desa Banteran, Kecamatan Sumbang, Kabupaten Banyumas, mulai September sampai dengan November 2015. Penelitian ini menggunakan Rancangan Acak Kelompok dengan 6 ulangan. Perlakuan terdiri atas kontrol, metabolit sekunder Trichoderma sp. isolat jahe, isolat bawang merah, gabungan isolat bawang merah dan isolat jahe, dan fungisida asam fosfat. Variabel yang diamati adalah masa inkubasi, intensitas penyakit, tinggi tanaman, jumlah daun, bobot tanaman segar dan kering, bobot akar segar dan kering, panjang akar, dan analisis senyawa fenol. Hasil penelitian menunjukkan bahwa perlakuan dengan menggunakan metabolit sekunder Trichoderma sp. isolat jahe mampu menekan intensitas penyakit 62,17%, dan mampu menunda masa inkubasi 24,97% dan perlakuan dengan menggunakan metabolit sekunder Trichoderma sp. isolat gabungan bawang merah dan jahe mampu meningkatkan pertambahan tinggi tanaman, jumlah daun, bobot tanaman segar, dan kering, bobot akar segar dan kering serta panjang akar masing-masing sebesar 36,21; 19,07; 20,6; 17,5; 27; 34,36; dan 21,30%. Perlakuan metabolit sekunder Trichoderma sp. mampu meningkatkan kandungan senyawa fenol (saponin, tanin, dan glikosida) pada bibit kakao.
Abtrak (Bhs. Inggris)This research aimed to study the secondary metabolite application of Trichoderma sp. from ginger and shallots isolates, or their combination on the disease and its effect on growth and on phenolic compound content of cocoa seedlings. This research was carried out at the Laboratory of Plant Protection, Faculty of Agriculture, Jenderal Soedirman University, Purwokerto and at Banteran Village, Sumbang Sub-District, Banyumas Regency, from September to November 2015. Randomized block design was used with nine replicates and five treatments consisted of control, secondary metabolit of Trichoderma sp. ginger and shallots isolates, their combination, and fungicide with active ingredient of phosphic acid. Variables observed were incubation period, disease intensity, plant height, number of leaves, wieght of fresh and dry plants, weight of fresh and dry roots, roots length, and phenolic compound content qualitatively. Result of the research showed that the secondary metabolit of Trichoderma sp. ginger isolate could suppress the disease intensity and delay the incubation period as 62.17 and 24.97%, respectively. The secondary metabolit treatment of Trichoderma sp. shallots and ginger combination could increase plant height, number of leaves, fresh weight of plant, dry weight of plant, fresh weight roots, dry weight of root, and roots length as 36.21, 19.07, 20.6, 17.5, 27, 34.36, and 21.30%, respectively. All secondary metabolites of Trichoderma sp. were able to increase phenolic compound content (saponins, tannins, and glycosides) qualitatively of cocoa seedlings.
Kata kunciBibit kakao, Metabolit sekunder Trichoderma sp., Penyakit pembuluh kayu
Pembimbing 1Prof. Ir. Loekas Soesanto, M.S., Ph.D.
Pembimbing 2Endang Mugiastuti, S.P., M.P.
Pembimbing 3
Tahun2016
Jumlah Halaman15
Tgl. Entri2016-04-25 17:13:22.378077
Cetak Bukti Unggah
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