Artikel Ilmiah : H1A012045 a.n. YUNASTIA PRATAMI
| NIM | H1A012045 |
|---|---|
| Namamhs | YUNASTIA PRATAMI |
| Judul Artikel | VALIDASI METODE ANALISIS KADAR GLUKOSA MENGGUNAKAN KAMERA HANDPHONE (TELEPON GENGGAM) |
| Abstrak (Bhs. Indonesia) | Dalam penelitian ini telah dikembangkan metode kolorimetri menggunakan pencitraan kamera handphone untuk menentukan kadar glukosa dan validasi metode tersebut. Penentuan glukosa secara kolorimetri didasarkan pada reaksi glukosa dengan enzim glukosa oksidase yang menghasilkan H2O2 yang kemudian bereaksi dengan KMnO4 sebagai indikator redoks. Perubahan warna KMnO4 diukur menggunakan kamera handphone dan dianalisis menggunakan program Color Grab. Validasi metode menunjukkan sensitivitas tertinggi pada intensitas warna hijau dengan nilai KV 1,682%; HORRAT 0,314; recovery 95,97%; rentang konsentrasi linear 0,5 mM sampai dengan 3,0 mM dengan nilai koefisien korelasi 0,9991. Batas deteksi (LOD) 0,13 mM; batas kuantifikasi (LOQ) 0,45 mM; rentang metode 0,45-38,78 mM. Selektivitas KMnO4 kurang selektif untuk menentukan H2O2 dengan adanya asam askorbat. Hasil analisis kadar glukosa dalam sampel menggunakan kamera handphone tidak berbeda secara signifikan dari hasil analisis menggunakan spektrofotometer UV-Vis (P>0,05). |
| Abtrak (Bhs. Inggris) | In this research a colorimetric method using a handphone camera imaging to determine the concentration of glucose and as method validation have been developed. The colorimetric glucose determination was based on the reaction of glucose with glucose oxidase enzyme resulted in H2O2 which was finally reacted with KMnO4 as redox indicators. The color change of KMnO4 was measured using a handphone camera and analyzed using Color Grab program. The method validation showed the highest sensitivity of the intensity was the green color with KV value of 1,682%; HORRAT of 0,314; recovery of 95,97%; linear range of 0,5 mM to 3,0 mM with a correlation coefficient of 0,9991. The limit of detection (LOD) was 0,13 mM; limit of quantification (LOQ) was 0,45 mM; methods range of 0,45–38,78 mM. The selectivity of KMnO4 was less selective to H2O2 determination in the presence of ascorbic acid. The results of the analysis of glucose levels in the samples using a handphone camera were not significantly different from the results of the analysis using UV-Vis spectrophotometer (P>0,05). |
| Kata kunci | analisis glukosa, kamera, handphone, kolorimetri, validasi metode |
| Pembimbing 1 | Dian Windy Dwiasi, M.Sc. |
| Pembimbing 2 | Amin Fatoni, Ph.D. |
| Pembimbing 3 | - |
| Tahun | 2016 |
| Jumlah Halaman | 60 |
| Tgl. Entri | 2016-04-22 16:43:09.720909 |