Artikel Ilmiah : H1A011053 a.n. IRMA NINGSIH
| NIM | H1A011053 |
|---|---|
| Namamhs | IRMA NINGSIH |
| Judul Artikel | Identifikasi Spesies Streptomyces K-2C dan K-4B Penghasil Zat Warna Alami |
| Abstrak (Bhs. Indonesia) | Streptomyces merupakan golongan dari aktinomisetes yang mampu menghasilkan pigmen warna dan beberapa enzim hidrolitik seperti, urease, amilase, protease, lipase, selulase dan nitrat reduktase. Streptomyces K-2C mampu menghasilkan gradasi warna merah dan K-4B gradasi warna ungu pada medium Strach Casein Nitrat (SCN). Tujuan dari penelitian ini yaitu untuk memperoleh identitas dari isolat Streptomyces K-2C dan K-4B yang memiliki kemampuan menghasilkan gradasi warna merah dan ungu serta mampu menghasilkan beberapa enzim hidrolitik, seperti urease, amilase, lipase, protease, selulase dan nitrat reduktase. Tahapan penelitian adalah kultivasi isolat Streptomyces K-2C dan K-4B pada medium Strach Casein Nitrat (SCN). Uji biokimiawi meliputi, uji urease, uji amilase, uji protease, uji lipase, uji selulase, uji reduksi nitrat. Isolasi DNA menggunakan PrestoTM Mini gDNA Bacteria Kit Geneaid. Amplifikasi DNA menggunakan PCR kit KAPA TaqTM Extra HotStart Ready Mix with dye. Elektroforesis DNA menggunakan gel agarosa 1% yang direndam dalam buffer TAE 1x. Hasil amplifikasi dianalisis sekuen fragmen 16S rRNA, kemudian ditentukan urutan basa dengan menggunakan pensejajaran program BioEdit. Penentuan spesies menggunakan program BLAST (Basic Local Alignment Search Tool) dan penentuan kekerabatan spesies Streptomyces K-2C dan K-4B menggunakan Multiple Alignment pada program BLAST. Hasil kultivasi isolat Streptomyces K-2C dan K-4B menunjukkan bahwa kedua isolat tersebut mampu menghasilkan gradasi warna merah dan ungu pada medium Strach Casein Nitrat (SCN). Hasil uji biokimiawi juga menunjukkan isolat Streptomyces K-2C dan K-4B mampu menghasilkan enzim urease, amilase, lipase, protease, selulase dan nitrat reduktase. Hasil pensejajaran melalui program BLAST menunjukkan kedua isolat memiliki kemiripan spesies terdekatnya sebesar 99% terhadap Streptomyces fradiae strain RSU15. Kedua isolat memiliki kesamaan urutan DNA sebesar 99% dan memiliki hubungan kekerabatan dalam pohon filogenik yang sangat dekat. |
| Abtrak (Bhs. Inggris) | Streptomyces is a group of actinomycetes capable of producing color pigments and some hydrolytic enzymes such as urease, amylase, protease, lipase, cellulase and nitrate reductase. Streptomyces K-2C is able to produce shades of red and K-4B shades of purple in the medium strach Casein Nitrate (SCN). The aim of this study is to identify the spesies Streptomyces K-2C and K-4B which have the ability to produce pigments red and purple as well as producing several hydrolytic enzymes, such as urease, amylase, lipase, protease, cellulase and nitrate reductase. Stages of research were the cultivation of Streptomyces isolates K-2C and K-4B on Strach Casein Nitrate (SCN) medium. Biochemical tests conducted were urease, amylase, protease, lipase, cellulase, and nitrate reduction tests. DNA isolation used PrestoTM Mini gDNA Bacteria Kit Geneaid. DNA amplification used PCR kit Extra HotStart KAPA TaqTM Ready Mix with dye. DNA electrophoresis used 1% agarose gel soaked in TAE 1x buffer. Amplification product was analyzed by which 16S rRNA fragments sequences, then was determined the sequence alignment with Bioedit program. Determination of species were done by BLAST (Basic Local Alignment Search Tool) program and the phylogenic tree of the species Streptomyces K-2C and K-4B were determinated with the Multiple Alignment on the BLAST program. The research results showed that Streptomyces isolates K-2C and K-4B were able to produce red and purple pigments in the Starch Casein Nitrate (SCN) medium. Biochemical test results showed that both Streptomyces K-2C and K-4B were able to produce the enzyme urease, amylase, lipase, protease, cellulase and nitrate reductase. The results from BLAST alignments program showed both isolates had the closest resemblance to species at 99% of the Streptomyces fradiae strain RSU15. Both isolates had DNA sequence similarity of 99% and had a very close alliance in filogenik tree. |
| Kata kunci | Streptomyces K-2C, Streptomyces K-4B, SCN, BLAST. |
| Pembimbing 1 | Ari Asnani, S.Si, M.Sc, Ph.D. |
| Pembimbing 2 | Dr. Hendro Pramono, M.S. |
| Pembimbing 3 | |
| Tahun | 2015 |
| Jumlah Halaman | 10 |
| Tgl. Entri | 2015-07-07 04:38:34.506047 |