Artikel Ilmiah : G1A011043 a.n. JATMIKO EDY NUGROHO
| NIM | G1A011043 |
|---|---|
| Namamhs | JATMIKO EDY NUGROHO |
| Judul Artikel | PERBEDAAN GAMBARAN HISTOLOGI HEPAR TIKUS PUTIH (Rattus Norvegicus) JANTAN PASCA PEMBERIAN EKSTRAK ETANOL DAUN SIRSAK (Annona muricata L.) Uji Toksisitas Subkronik Ekstrak Etanol Daun Sirsak |
| Abstrak (Bhs. Indonesia) | Latar Belakang : Daun sirsak banyak digunakan sebagai obat herbal karena terbukti berpotensi sebagai antioksidan, kemopreventif, serta antikanker. Kerusakan hepar dapat terjadi akibat konsumsi obat herbal per oral dalam waktu jangka panjang. Keamanan daun sisak terhadap hepar bisa diketahui dari hasil uji toksisitas. Tujuan : Mengetahui perbedaan gambaran histologi hepar tikus putih jantan pasca pemberian ekstrak etanol daun sirsak berbagai dosis secara subkronik. Metode : Penelitian eksperimental dengan post test only with control group design. Tikus putih jantan (30 ekor) dibagi dalam 5 kelompok. Kelompok A (Tween 80 1,75% dalam 3 ml aquades), kelompok B (ekstrak etanol daun sirsak 75 mg/kgBB), kelompok C (ekstrak etanol daun sirsak 150 mg/kgBB) kelompok D (ekstrak etanol daun sirsak 300 mg/kgBB), dan kelompok E (ekstrak etanol daun sirsak 450 mg/kgBB) yang diberikan selama 28 hari. Hepar diambil dan dibuat sediaan histologi dengan pewarnaan HE dan dinilai menggunakan modifikasi sistem penilaian Scheuer. Hasil : Hasil rerata skor kerusakan hepar kelompok A 3,1±0,23; kelompok B 3,0±0,14; kelompok C 3,0±0,15; kelompok D 2,9±0,16; dan kelompok E 2,8±0,23. Uji ANOVA menunjukkan perbedaan signifikan (p=0,022), dilanjutkan Post Hoc LSD menunjukkan perbedaan signifikan (p<0,05) antara kelompok E dengan kelompok A (kontrol). Kesimpulan : Terdapat perbedaan skor kerusakan gambaran histologi hepar tikus putih jantan pada kelompok dosis 450 mg/kgBB yang secara signifikan lebih rendah daripada kelompok kontrol. |
| Abtrak (Bhs. Inggris) | Background : Soursop leaves had widely consumed as herbal medicine because it’s potential as antioxidants, chemopreventive and anticancer. Liver damage potentially happened because of the long term consumption of herbal medicine. The soursoup leaves safety in liver histology condition can been known by the toxicity test. Objective : This study aimed to determine differences in male albino rats liver histological changes after various doses of subchronic soursop leaf ethanolic extract (SLE) administration. Method : Research method was experimental study with post test only with control group design. Thirty male albino rats were divided into 5 groups. Group A received Tween 80 1,75% in 3 ml of aquades, group B SLE 75 mg/KgWeight, group C SLE 150 mg/KgWeight, group D SLE 300 mg/KgWeight, and group E SLE 450 mg/KgWeight for 28 days. The Liver was taken and made histological preparations with HE staining and assesed by the modification of Scheuer score. Result : The result of liver histological damage mean score in group A was 3,1±0,23; group B was 3,0±0,14; group C was 3,0±0,15; group D was 2,9±0,16; and group E was 2,8±0,23. ANOVA test showed significant differences (p=0.022), post hoc LSD showed a significant difference (p < 0.05) between groups E with control. Conclusion : There was a significant difference of damage score in liver histologic changes of male albino rats in control group which was higher than 450 mg/KgWeight dosage group. |
| Kata kunci | uji toksisitas, daun sirsak, kerusakan hepar, piecemeal necrosis, Skor Scheuer |
| Pembimbing 1 | dr.Evy Sulistyoningrum, M.Sc |
| Pembimbing 2 | Dr.dr.Eman Sutrisna, M.Kes |
| Pembimbing 3 | |
| Tahun | 2015 |
| Jumlah Halaman | 11 |
| Tgl. Entri | 2015-06-19 09:34:41.670145 |