Artikel Ilmiah : B1J011159 a.n. NUR FITRIANTO
| NIM | B1J011159 |
|---|---|
| Namamhs | NUR FITRIANTO |
| Judul Artikel | STUDI KARAKTER ANATOMI DAUN DAN ANALISIS KANDUNGAN KLOROFIL BEBERAPA KULTIVAR UBI JALAR (Ipomoea batatas L.) |
| Abstrak (Bhs. Indonesia) | Penelitian ini berjudul “Studi Karakter Anatomi Daun dan Analisis Kandungan Klorofil Beberapa Kultivar Ubi Jalar (Ipomoea batatas L.)”, bertujuan mengetahui perbedaan struktur dan karakter anatomi daun serta kandungan klorofil beberapa kultivar Ipomoea batatas L.. Metode penelitian survai dengan teknik pengambilan sampel acak terpilih (purposive random sampling) di wilayah Purwokerto dan sekitarnya. Pembuatan preparat anatomi daun menggunakan metode parafin dengan pewarnaan safranin 1% dalam alkohol 70% dan spektrofotometer untuk mengukur kandungan klorofil. Variabel berupa karakter anatomi dan kandungan klorofil daun, dengan parameter tebal kutikula dan mesofil, ukuran panjang dan lebar stomata, kerapatan stomata dan trikomata per mm², rasio palisade, dan kandungan klorofil. Analisis data secara deskriptif untuk menginterpretasikan perbedaan struktur dan karakter anatomi serta kandungan klorofil. Berdasarkan hasil survai didapatkan sepuluh kultivar I. batatas L. yaitu I. batatas L. ‘Antin’, ‘Beta’, ‘Borobudur’, ‘Cangkuang’, ‘Cilembu’, ‘Kidal’, ‘Sari’, ‘Solosa Kuning’, ‘Sukuh’ dan ‘Ungu Tua’. Kesepuluh kultivar memiliki struktur anatomi daun yang seragam. Karakter anatomi pada kesepuluh kultivar diperoleh hasil beragam. Kutikula paling tebal pada ‘Cangkuang’ 4,25 µm, mesofil paling tebal pada ‘Cilembu’ 175,5 µm. Panjang dan lebar stomata paling tinggi Ungu tua pada permukaan adaksial 30,5 µm dan 5 µm, pada permukaan abaksial paling panjang pada Antin 35,5 µm dan paling lebar pada ‘Borobudur’ dan ‘Sukuh’ 4,75 µm. Kerapatan stomata paling tinggi di permukaan adaksial pada ‘Cangkuang’ dan ‘Sukuh’ yaitu 8 sel/mm2, di permukaan abaksial pada ‘Borobudur’ yaitu 17 sel/mm2. Kerapatan trikomata pada permukaan adaksial antara 1-2 sel/mm2, pada abaksial paling tinggi ‘Beta’ yaitu 3 sel/mm2. Rasio palisade per 4 sel epidermis didapatkan jumlah sel palisade tertinggi pada ‘Cilembu’ yaitu 13 sel. Analisis kandungan klorofil a, klorofil b, dan klorofil total tertinggi yaitu pada ‘Cilembu’ sebesar 1,44 mg.l-1, 0,725 mg.l-1, dan 1,787 mg.l-1. |
| Abtrak (Bhs. Inggris) | A research entitled “Study of Leaf Anatomy Character and Analysis Chlorophyll Content on Sweet Potato Cultivars (Ipomoea batatas L.)”, aimed to determine the differentiation of structural and anatomical characters of leaf and chlorophyll contents in sweet potato cultivars. The method of this research was survey with purposive random sampling at Purwokerto arounds. Leaf anatomy slide were made by using paraffin method and were dyed with 1% of safranine in 70% of alcohol and spectrophotometry method was used to determine the content of chlorophyll. Variables observed were leaf anatomical characters and chlorophyll content of sweet potato cultivars, with measured parameters were cuticule and mesophyl thickness, length and wide of stomata, stomata and trichome density per mm2, palisade ratio, and leaf chlorophyll content. Data analysed descriptively to interpret leaf different structure, anatomical character, and chlorophyll content. The results of this research were ten cultivars of sweet potato i.e., I. batatas L. ‘Antin’, ‘Beta’, ‘Borobudur’, ‘Cangkuang’, ‘Cilembu’, ‘Kidal’, ‘Sari’, ‘Solosa Kuning’, ‘Sukuh’ and ‘Ungu Tua’. Those cultivars have the same or uniform anatomical structure. Anatomical characters of those cultivars were varied in size and cell number in particular character. The highest cuticule thickness was in ‘Cangkuang’ with 4,25 µm. The biggest stomata density in adaksial surface were in ‘Cangkuang’ and ‘Sukuh’ with 8 cell/mm2, meanwhile in abaksial surface was in ‘Borobudur’ with 17 cell/mm2. Trichome density in adaksial surface ranged from 1 to 2 cell/mm2, meanwhile the highest density in abaksial surface was in ‘Beta’ with 3 cell/mm2. The greatest palisade cell number was in ‘Cilembu’ with 13 cells out of 4 epidermis cells palisade ratio. The highest chlorophyl content was in ‘Cilembu’ with 1,44 mg.l-1 for chlorophyl a; 0,725 mg.l-1 for chlorophyl b; and 1,787 mg.l-1 for total chlorophyl. Keywords: Ipomoea batatas L., cultivar, characters of leaf anatomy, analysis of chlorophyll |
| Kata kunci | Ipomoea batatas L., kultivar, karakter anatomi daun, analisis klorofil |
| Pembimbing 1 | Dra. Siti Samiyarsih, M.Si |
| Pembimbing 2 | Drs. Rochmatino, M.Si |
| Pembimbing 3 | |
| Tahun | 2015 |
| Jumlah Halaman | 10 |
| Tgl. Entri | 2015-05-07 13:42:47.699566 |