| NIM | P2BA12008 |
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| Namamhs | INDAH MASTUTI, S.Si |
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| Judul Artikel | REAKTIVITAS ANTISERUM MENCIT TERHADAP PROTEIN KAPSID MAYOR GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV) GENUS MEGALOCYTIVIRUS |
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| Abstrak (Bhs. Indonesia) | Megalocytivirus merupakan salah satu virus yang sering menyebabkan kerugian dalam budidaya perikanan. Metode deteksi cepat diperlukan untuk mendukung upaya pencegahan infeksi virus ini. Imunobloting merupakan salah satu teknik sederhana yang dapat diterapkan untuk deteksi virus secara cepat. Penelitian ini menggunakan rekombinan protein kapsid mayor virus grouper sleepy disease iridovirus (rMCP-GSDIV) sebagai antigen untuk menginduksi pembentukan antibodi pada mencit. Protein rMCP-GSDIV diduga dapat menginduksi pembentukan antibodi tanpa menggunakan adjuvant. Berdasarkan hipotesis tersebut dilakukan penelitian induksi antiserum terhadap rMCP-GSDIV dengan menggunakan adjuvant (emulsi 50 µg/µl rMCP-GSDIV + Freund’s adjuvant , 1:1 v/v) dan tanpa adjuvant (suspensi 50 µg/µl rMCP-GSDIV dan akuabidest, 1:1 v/v). Imunisasi mencit dilakukan sebanyak dua kali dengan interval waktu 14 hari melalui injeksi intramuskular. Antiserum yang diperoleh menunjukkan reaktivitas terhadap rMCP-GSDIV hingga pengenceran 1/32. Reaktivitas pada tiap pengenceran dianalisis dengan uji Kappa. Antiserum yang diperoleh pada 14 hari setelah imunisasi pertama (dengan atau tanpa adjuvant) menunjukkan kesamaan reaktivitas (83.33%) dengan nilai Kappa 0,22. Demikian pula antiserum yang diperoleh pada 14 hari setelah imunisasi kedua menunjukkan kesamaan reaktivitas (95,83%) dengan nilai Kappa 0. Berdasarkan hasil tersebut dapat disimpulkan bahwa antiserum yang reaktif terhadap rMCP-GSDIV dapat diperoleh dengan mengimunisasi mencit menggunakan adjuvant atau tanpa adjuvant. |
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| Abtrak (Bhs. Inggris) | Megalocytivirus is the main cause of aquaculture loss in Asia including Indonesia. Early detection of this viral infection is nesessary. In accordance to this, a fast reliable method to detect viral infection is paramount. Immunoblotting is one of the favourable choices because it is simple yet reliable. In this study a recombinant Mayor Capsid Protein of Grouper Sleepy Disease Iridivirus (rMCP-GSDIV) was used as antigen to induce antibody production in mice. Considering that the protein size is big and is conjugated to tag protein, is was hypothesised that this protein capable of inducing antibody production without adjuvant. To proove this hypothesis two groups of 6 mice were immunised with emultion 50µg/µl rMCP-GSDIV and Freund’d adjuvant (ratio 1:1 v/v) and suspension of rMCP-GSDIV and aquabidest (ratio 1:1 v/v). Immunisation was given twice with 14 days interval. The mice were bleed from orbitalis vein for antisera collection. The antisera were tested for their immunoreactivity in serial dillutions up to 1/32 using dot blott on a PVDF membrane. The reactivity of each dillution level was analysed using Kappa. The results showed that rMCP-GSDIV either with or without adjuvant are capable of inducing reactive antisera at 1/32 dilution. The antisera obtained from rMCP-GSDIV induced mice (either with or without adjuvant) at 14 days post first induction showed similar reactivity (83.33%) with kappa value = 0.22. The antisera obtained at 14 days post second induction produced reactivity of 95.83% with kappa value = 0. These results suggested that a reactive antiserum against rMCP-GSDIV could be produced by immunizizing mice with the protein with or without addition of adjuvant. |
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| Kata kunci | Megalocytivirus, protein kapsid mayor, immunoblotting |
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| Pembimbing 1 | Dra. G.E. Wijayanti, M.Rep.Sc, Ph.D |
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| Pembimbing 2 | Dr. Hemayanti |
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| Pembimbing 3 | |
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| Tahun | 2014 |
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| Jumlah Halaman | 15 |
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| Tgl. Entri | 2015-02-25 15:05:23.560574 |
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