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UJI KEMAMPUAN MIKROBA ANTAGONIS UNTUK MENGENDALIKAN PENYAKIT LAYU BAKTERI PADA TANAMAN KENTANG
Abstrak (Bhs. Indonesia)
Penelitian ini bertujuan untuk mengetahui pengaruh mikroba antagonis dalam mengendalikan penyakit layu bakteri serta pengaruhnya terhadap pertumbuhan dan hasil tanaman kentang. Penelitian dilaksanakan dari bulan Maret sampai Juni 2014, di Laboratorium Perlindungan Tanaman Fakultas Pertanian Universitas Jenderal Soedirman dan di screen house Desa Serang, Kecamatan Karangreja, Kabupaten Purbalingga. Penelitian menggunakan Rancangan Acak Kelompok dengan 6 perlakuan dan 4 ulangan. Perlakuan yang dicoba meliputi: kontrol, P. fluorescens P60, P. fluorescens P60+P8, P. fluorescens P60+T. harzianum, P. fluorescens P60+P8+T. harzianum, dan bakterisida. Variabel yang diamati meliputi masa inkubasi, intensitas penyakit, kepadatan akhir patogen dan antagonis, tinggi tanaman, jumlah daun per tanaman, jumlah cabang per tanaman, bobot segar dan kering tanaman, bobot segar dan kering akar, jumlah umbi, bobot umbi, dan analisis senyawa fenol. Hasil penelitian menunjukkan bahwa mikroba antagonis belum mampu menunda masa inkubasi dan menekan intensitas penyakit layu bakteri pada tanaman kentang. Perlakuan gabungan P. fluorescens P60+P8+T. harzianum mampu meningkatkan tinggi tanaman sebesar 33,87% dan jumlah umbi sebesar 32%, namun belum mampu meningkatkan jumlah daun per tanaman, jumlah cabang pertanaman, bobot segar dan kering tanaman, bobot segar dan kering akar serta bobot umbi.
Abtrak (Bhs. Inggris)
This research was aimed to know the effectiveness of microbial antagonists in controlling bacterial wilt disease and its influence on the growth and yield of potato. The research was carried out at the Plant Protection Laboratory of Agriculture Faculty of Jenderal Soedirman University, Purwokerto and in the screen house at Serang Village, Subdistrict Karangreja, Purbalingga Regency from March to June 2014. Randomized Block Design was used in the research with six treatments and four replications. The treatments tested include: control, P. fluorescens, P. fluorescens P60+P8, P. fluorescens P60+T. harzianum, P. fluorescens P60+P8+T. harzianum, and bactericide. Variables observed were incubation period, disease intensity, final density of the pathogen and the antagonistic microbes, plant height, number of leaves per plants, number of branch per plants, fresh and dry weight of plants, fresh and dry of roots, number of tubers, tubers weight, and analysis of phenolic compounds. The results showed that the antagonistic microbes have not been able to delay the incubation period and have not been able to suppress bacterial wilt disease intensity on potato. The combination treatments of P. fluorescens P60+P8+T. harzianum was able to increase plant height as 33,87% and number of tubers as 32%, but the antagonistic microbes have not been able to increase the number of leaves per plants, number of branch per plants, fresh and dry weight of plants, fresh and dry of roots, and tubers weight.
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