Update Artikelilmiah: 7831

MITHA MAULIDYA

NIM

Judul Artikel

Abstrak (Bhs. Indonesia)

Syarat mikrobiologis dengan parameter total Coliform untuk air bersih sesuai peraturan Menkes No. 416/Menkes/per/IX/1990 adalah 10 jumlah Coliform per 100ml (air perpipaan) dan 50 jumlah Coliform per 100ml (air bukan perpipaan). Tujuan penelitian ini untuk mengetahui ada tidaknya dan jumlah bakteri Coliform serta mengidentifikasi bakteri tersebut pada air sumur warga di Desa Pamijen Kecamatan Sokaraja Kabupaten Banyumas.
Penelitian ini dilakukan dengan metode observasional. Pengujian Coliform terdiri dari uji pendugaan, uji konfirmasi, dan uji kelengkapan. Media Lactose Broth (LB) dengan seri tabung 333 digunakan untuk melihat kekeruhan dan terbentuknya gas, media Eosin Methylen Blue Agar (EMBA) digunakan untuk mengkonfirmasi pertumbuhan bakteri Gram negatif sedangkan  media Triple Sugar Iron Agar (TSIA) digunakan untuk identifikasi bakteri. Perhitungan jumlah bakteri Coliform menggunakan tabel MPN (Most Probable Number). 
Analisis data ditampilkan secara deskriptif. Hasil penelitian ini menunjukan bahwa sampel air sumur di Desa Pamijen tercemar Coliform jumlah Coliform total tidak memenuhi persyaratan Menkes, yaitu terdapat pada sumur 3,4,5 adalah 210/100 ml, sumur 6 adalah 150/100 ml, sumur 7 adalah 120/100 ml, sumur 8 adalah 93/100 ml , dan didapatkan 6 isolat Coliform fekal yaitu Shigella, Escherichia coli, Proteus vulgaris, Enterobacter, Pseudomonas, Salmonella dan 1 isolat Coliform non-fekal yaitu Enterobacter aerogenes.

Abtrak (Bhs. Inggris)

The total Coliform requirement base on the Menkes regulation No. 416/Menkes/per/IX/1990 is 10 numbers of Coliform per 100 ml for piping water and 50 numbers of Coliform per 100 ml for no piping water. The purpose of this research was to find out there was any coliform bacteria and to find out the number of Coliform bacteria in well water in the Pamijen village, Sokaraja Regency, Banyumas district and also identified by the genus or may be the species of bacteria. 
The research was conducted observational method. Coliform testing consists of presumptive test, confirmed test, and completed test. Lactose Broth (LB) medium  by 333 series tube was used to see the cloudiness and gas formation, Eosin Methylen Blue Agar (EMBA) medium was used to confirm the growth of Gram-negative bacteria while Triple Sugar Iron Agar (TSIA) medium was used to identify the bacteria. Calculation the amount of coliform bacteria use MPN (Most Probable Number) table. 
The data was analysed using the descriptive method. The results of this research showed that the sample of well water in the village of Pamijen was contaminated by Coliform and total Coliform didn’t meet the requirement Menkes, found at the  third, fourth, and fifth well were 210/100 ml,  the sixth well was 150/100 ml, the seventh well was 120/100 ml, the eighth well was  93/100 ml, and it was found  6 isolates of Coliform fekal, namely Shigella, Escherichia coli, Proteus vulgaris, Enterobacter, Pseudomonas, Salmonella and 1 non-fekal Coliform isolates namely Enterobacter aerogenes.

Kata kunci

Pembimbing 1

Pembimbing 2

Pembimbing 3

Tahun

Jumlah Halaman