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ELLY CINDY NAYA ARDITA

NIM

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Abstrak (Bhs. Indonesia)

Penelitian telah  dilakukan untuk mengetahui  senyawa  utama,  karakteristik,  aktivitas
antibakteri  dan  Kosentrasi  Hambat  Tumbuh  Minimum  (KHTM)  minyak  atsiri  fuli  pala  dariBanyumas  terhadap  bakteri E.  coli. Fuli  pala  ditentukan  kadar  airnya dan didestilasi  air. Minyak  atsiri  ditentukan  rendemen  dan  sifat  fisik  (densitas, indeks  bias,  dan  putaran  optik) serta diidentifikasi  senyawa  utamanya  dengan  GC-MS. Minyak  atsiri  fuli  diuji  aktivitasantibakteri dan ditentukan KHTM  dengan metode  difusi. Fuli  mempunyai  kadar  air  7.52% dan minyak atsiri fuli yang diperoleh memiliki rendemen 27.80%. Densitas, indeks bias, danputaran  optik minyak  atsiri  fuli secara  berurutan  adalah 0.9276  g/cm3, 1.4891,  dan +12.25o. Hasil  identifikasi  dengan  GC-MS  diperoleh  5  komponen  utama  yaitu  myristisin  (25.05%), sabinen  (11.82%),  terpinen-4-ol  (10.41%), alfa-pinen  (7.29%),  dan  beta-pinen  (7.23%).  Minyak atsiri  fuli terbukti  memiliki  aktivitas  antibakteri.  Pada  konsentrasi  25%  v/v  diameter  zonajernihnya 12.12  mm  dan  pada  tetrasiklin  25%  v/v  dihasilkan zona  jernih  26.86 mm yangmenunjukkan  aktivitas  antibakteri  minyak  atsiri  fuli  lebih  rendah  dibanding  tetrasiklin.KHTM minyak atsiri fuli 0.75% v/v dengan diameter zona jernih 0.015 mm.

Abtrak (Bhs. Inggris)

The  research has  been  done to  find  out  the  main  compound,  characteristics,
antibacterial  activities and  Minimum Growth  Resistance Concentration  (KHTM)  of  nutmeg mace essential oils Banyumas foward bacteria E. coli. Nutmeg mace is determined its water contents and distilled water. Essential oil is determined the rendemen and physical properties (density, refractive index, and optical rotation) as well as the main compounds identified by GC-MS. Mace  essential  oils  are  tested  antibacterial  activity  and KHTM is  determined with diffusion  method. Mace has  a  7.52%  water  content  and  essential  oil  obtained  mace  has  a 27.80% rendemen. Density, refractive index, and optical rotation which is obtain by GC-MS is  mace  essential  oil  as chronological  is 0.9276  g/cm3,  1.4891,  and  +12.25o.  Identification results with GC-MS obtained 5  main  components  myristisin  (25.05%),  sabinen  (11.82%), terpinen-4-ol  (10.41%),  α-pinene  (7.29%),  and  β-pinene  (7.23%).  Mace  essential  oil  has proven its antibacterial activity. In concentration of 25% v/v the diameter of the clear zone is 12.12  mm  and in  the  tetracycline  25%  v/v is produced  a  clear  zone  of  26.86  mm  which
showed  antibacterial  activity  of mace essential  oil  lower  than  tetracycline.  KHTM  mace essential oil 0.75% v/v with clear zone diameter is 0.015 mm.

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