Update Artikelilmiah: 51115

RYAN BRIAN HAFIYAN PUTRA

NIM

Judul Artikel

Abstrak (Bhs. Indonesia)

Latar Belakang: Candida tropicalis merupakan patogen oportunistik yang mampu membentuk biofilm sehingga meningkatkan resistensi terhadap antijamur. Nanopartikel perak (AgNP) berbasis ekstrak etil asetat terong ungu (Solanum melongena L.) diperkirakan memiliki aktivitas antijamur dan antibiofilm lebih baik karena ukuran partikel kecil meningkatkan penetrasi dan pelepasan ion Ag⁺ yang bersifat toksik bagi sel jamur.. Tujuan: Mengetahui aktivitas antijamur dan antibiofilm nanopartikel perak ekstrak etil asetat terong ungu (Solanum melongena L.)  terhadap Candida tropicalis in vitro, melalui penentuan nilai Konsentrasi Hambat Minimal (KHM), Konsentrasi Bunuh Minimal (KBM), Minimum Biofilm Inhibitory Concentration 50% (MBIC₅₀), dan Minimum Biofilm Reduction Concentration 50% (MBRC₅₀). Metode: Penelitian true experimental dengan desain post test only control group design. KHM ditentukan menggunakan mikrodilusi, sedangkan KBM melalui metode spread plate. Aktivitas antibiofilm diukur dengan microtiter plate assay untuk menentukan MBIC₅₀ dan MBRC₅₀. Kontrol meliputi kontrol negatif, kontrol positif (flukonazol 6,25 µg/mL), kontrol media, dan kontrol pelarut. Perlakuan terdiri dari konsentrasi AgNP 4, 2, 1, 0.5 dan 0.25 mg/mL. Hasil: AgNP ekstrak etil asetat terong ungu menunjukkan aktivitas penghambatan pertumbuhan dengan KHM pada konsentrasi 4 mg/mL. Persentase daya bunuh jamur terbesar adalah 99,18%, namun belum memenuhi kriteria KBM ≥99,99%, sehingga KBM tidak diperoleh. Aktivitas antibiofilm terdeteksi dengan MBIC₅₀ dan MBRC₅₀ pada konsentrasi 0,25 mg/mL, menunjukkan kemampuan menghambat pembentukan serta mengurangi biofilm pada konsentrasi rendah. Kesimpulan: : AgNP ekstrak etil asetat terong ungu berpotensi sebagai agen antijamur dan antibiofilm terhadap C. tropicalis, meskipun belum menunjukkan aktivitas fungisidal berdasarkan kriteria KBM.

Abtrak (Bhs. Inggris)

Background: Candida tropicalis is an opportunistic pathogen capable of forming biofilms, which increase its resistance to antifungal agents. Silver nanoparticles (AgNPs) derived from ethyl acetate extract of purple eggplant (Solanum melongena L.) are predicted to have enhanced antifungal and antibiofilm activity due to their small particle size, which facilitates penetration and the release of toxic Ag⁺ ions into fungal cells. Objective: To determine the antifungal and antibiofilm activity of silver nanoparticles synthesized from ethyl acetate extract of Solanum melongena L. against C. tropicalis in vitro, through the evaluation of Minimum Inhibitory Concentration (MIC), Minimum Fungicidal Concentration (MFC), Minimum Biofilm Inhibitory Concentration 50% (MBIC₅₀), and Minimum Biofilm Reduction Concentration 50% (MBRC₅₀). Methods: This true experimental study used a post-test only control group design. MIC was determined using a microdilution method, while MFC was assessed using the spread plate method. Antibiofilm activity was evaluated using a microtiter plate assay to determine MBIC₅₀ and MBRC₅₀. The control groups included a negative control, a positive control (fluconazole 6.25 µg/mL), a media control, and a solvent control. Treatment groups consisted of AgNP concentrations of 4, 2, 1, 0.5, and 0.25 mg/mL. Results: AgNPs from ethyl acetate extract of purple eggplant inhibited fungal growth with an MIC of 4 mg/mL. The highest percentage of fungal killing was 99.18%, which did not meet the MFC criterion of ≥99.99%, indicating that a MFC was not achieved. Antibiofilm activity was observed with MBIC₅₀ and MBRC₅₀ values at 0.25 mg/mL, demonstrating the nanoparticles ability to inhibit and reduce biofilm formation at low concentrations. Conclusion: AgNPs derived from Solanum melongena L. exhibit potential antifungal and antibiofilm agents against C. tropicalis, although fungicidal activity based on MFC criteria was not achieved.

Kata kunci

Pembimbing 1

Pembimbing 2

Pembimbing 3

Tahun

Jumlah Halaman