Update Artikelilmiah: 49741

MUHAMMAD RIJALUL FIKRI

NIM

Judul Artikel

Abstrak (Bhs. Indonesia)

Penelitian ini bertujuan untuk mengetahui potensi aktivitas antimikroba minyak 
essential daun cengkeh sebagai larutan teat dipping serta menentukan konsentrasi terbaik berdasarkan uji zona hambat dan uji MIC terhadap khamir Candida albicans dan Saccharomyces cerevisiae sebagai penyebab mastitis mikotik. Uji zona hambat dilakukan menggunakan metode difusi cakram (paper disc), sedangkan uji MIC menggunakan metode mikrodilusi (broth microdilution). Rancangan percobaan zona hambat menggunakan Rancangan Acak Lengkap (RAL) dengan enam perlakuan dan tiga ulangan, terdiri atas kontrol positif (povidone iodine 10%), kontrol negatif (Tween 80 v/v 5%), serta minyak 
essential daun cengkeh dengan konsentrasi 2,5%, 5%, 7,5%, dan 10%. Uji MIC menggunakan Uji Mann-Whitney berdasarkan konsentrasi terbaik minyak essential dan povidone iodine 10%, masing-masing tiga ulangan. Hasil penelitian menunjukkan bahwa minyak essential daun cengkeh memiliki aktivitas antimikroba terhadap kedua khamir penyebab mastitis. Analisis variansi menunjukkan perlakuan memberikan pengaruh sangat nyata (p < 0,01). 
Zona hambat Candida albicans pada konsentrasi 0–10% berturut-turut adalah 0 mm, 5,55 mm, 8,23 mm, 10,23 mm, dan 12,85 mm, sedangkan povidone iodine 10% sebesar 28,85 mm. Zona hambat Saccharomyces cerevisiae pada povidone iodine 10% sebesar 22,73 mm, dan pada minyak essential 10–0% berturut-turut sebesar 20,46 mm, 17,23 mm, 14,27 mm, 7,23 mm, dan 0 mm. MIC terhadap kedua khamir masing-masing sebesar 0,156 mg/ml, dan povidone iodine 10% sebesar 1,25 mg/ml. Uji Mann-Whitney menunjukkan  perbedaan yang signifikan (p<0,05) antara perlakuan minyak cengkeh 10% dan povidone iodine 10% pada kedua khamir, sehingga minyak essential daun cengkeh dapat menggantikan povidone iodine 10% sebagai larutan teat dipping alami.

Abtrak (Bhs. Inggris)

This study aimed to evaluate the antimicrobial potential of clove leaf essential oil as a teat dipping solution and to determine the optimal concentration through inhibition zone and MIC tests against Candida albicans and Saccharomyces cerevisiae, which are fungal pathogens causing mycotic mastitis. The disc diffusion method was used for the inhibition zone test with a Completely Randomized Design (CRD) consisting of six treatments and three replications, namely positive control (10% povidone iodine), negative control (5% Tween 80 v/v), and clove leaf essential oil at concentrations of 2.5%, 5%, 7.5%, and 10%. The MIC test was conducted using the broth microdilution method with the best concentration. The results showed that clove leaf essential oil exhibited significant antimicrobial activity (p<0.01). The inhibition zones for Candida albicans at concentrations of 0–10% were 0 mm, 5.55 mm, 8.23 mm, 10.23 mm, and 12.85 mm, while 10% povidone iodine reached 28.85 mm. The inhibition zones for Saccharomyces cerevisiae at 10% povidone iodine were 22.73 mm, and for clove oil from 10–0% were 20.46 mm, 17.23 mm, 14.27 mm, 7.23 mm, and 0 mm, respectively. The MIC of clove leaf essential oil against both yeasts was 0.156 mg/ml, lower than 10% povidone iodine (1.25 mg/ml). The MannWhitney test showed a significant difference (p<0.05) between 10% clove oil and 10% povidone iodine treatments, indicating that clove leaf essential oil has the potential to replace povidone iodine as a natural teat dipping solution.

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