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TIO ARDHANA

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Abstrak (Bhs. Indonesia)

Penelitian ini bertujuan untuk mengetahui potensi Clove Leaf Essential Oil (CLEO) sebagai larutan teat dipping berdasarkan aktivitas antimikroba terhadap bakteri utama penyebab mastitis, yaitu Staphylococcus aureus dan Escherichia coli. Penelitian dilakukan melalui uji zona hambat dengan metode Disk Diffusion dan Konsentrasi Hambat Minimal (KHM) dengan metode Broth Microdilution. Penelitian menggunakan kultur bakteri Staphylococcus aureus ATCC 6538 dan Escherichia coli ATCC 25922. Rancangan percobaan uji zona hambat menggunakan Rancangan Acak Lengkap (RAL) dengan enam perlakuan dan tiga ulangan, menghasilkan total 18 unit percobaan untuk masing-masing bakteri. Perlakuan meliputi Kontrol (povidone iodine 10%), P0 (tween 80 5%), P1 (CLEO 2,5%), P2 (CLEO 5%), P3 (CLEO 7,5%), P4 (CLEO 10). Uji Mann Whitney untuk membandingkan antara Konsentrasi Hambat Minimal (KHM) CLEO dan povidone iodine dengan masing-masing tiga ulangan terhadap Staphylococcus aureus ATCC 6538 dan Escherichia coli ATCC 25922. Hasil Anova menunjukkan bahwa perlakuan berpengaruh sangat nyata (P<0,01) terhadap zona hambat Staphylococcus aureus ATCC 6538 dan Escherichia coli ATCC 25922. P4 (CLEO 10%) merupakan perlakuan terbaik yang berbeda nyata (P<0,05) dengan Kontrol (povidone iodine 10%) terhadap Staphylococcus aureus ATCC 6538 dan tidak berbeda nyata (P>0,05) dengan Kontrol (povidone iodine 10%) terhadap Escherichia coli ATCC 25922. Konsentrasi Hambat Minimal (KHM) CLEO didapatkan pada angka 0,31%, sedangkan povidone iodine pada 2,5% terhadap Staphylococcus aureus ATCC 6538 dan Escherichia coli ATCC 25922. Hasil Uji Mann Whitney menunjukkan bahwa CLEO memiliki nilai KHM lebih rendah (P<0,05) dibandingkan povidone iodine terhadap Staphylococcus aureus ATCC 6538 dan Escherichia coli ATCC 25922.

Abtrak (Bhs. Inggris)

This study aimed to determine the potential of Clove Leaf Essential Oil (CLEO) as a teat dipping solution based on its antimicrobial activity against the main bacteria causing mastitis, namely Staphylococcus aureus and Escherichia coli. The research was conducted using an inhibition zone test with the Disk Diffusion method and Minimum Inhibitory Concentration (MIC) test with the Broth Microdilution method. The bacterial cultures used were Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 25922. The inhibition zone test employed a Completely Randomized Design (CRD) with six treatments and three replications, resulting in a total of 18 experimental units for each bacterium. The treatments included Control (povidone iodine 10%), P0 (Tween 80 5%), P1 (CLEO 2,5%), P2 (CLEO 5%), P3 (CLEO 7,5%), and P4 (CLEO 10%). The Mann Whitney test was used to compare the Minimum Inhibitory Concentration (MIC) values of CLEO and povidone iodine, each with three replications, against Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 25922. Anova results showed that treatments had a highly significant effect (P<0,01) on the inhibition zones of both Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 25922. P4 (CLEO 10%) was the best treatment, showing a significant difference (P<0,05) compared to the Control (povidone iodine 10%) against Staphylococcus aureus ATCC 6538, and no significant difference (P>0,05) compared to the Control against Escherichia coli ATCC 25922. The Minimum Inhibitory Concentration (MIC) of CLEO was found to be 0,31%, while povidone iodine was 2,5% against both Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 25922. The MannWhitney test results indicated that CLEO had a significantly lower Minimum Inhibitory Concentration (MIC) value (P<0,05) than povidone iodine against both Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 25922.

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