Update Artikelilmiah: 49460

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Abstrak (Bhs. Indonesia)

Penyakit degeneratif yang disebabkan oleh radikal bebas memunculkan 
pengembangan senyawa antioksidan dan antiinflamasi alami, salah satunya dari 
peptida bioaktif. Peptida bioaktif dapat diperoleh salah satunya melalui hidrolisis 
enzimatik menggunakan protein ikan lele (Clarias gariepinus). Tahapan penelitian 
meliputi isolasi protein ikan lele, produksi ekstrak kasar enzim protease dari bakteri 
Bacillus subtilis B209, serta karakterisasi suhu dan pH. Protein ikan lele dihidrolisis 
menggunakan ekstrak kasar enzim variasi waktu 10, 20, 30, 40, 50, dan 60 menit 
pada kondisi optimum dan ditentukan nilai derajat hidrolisis serta diuji aktivitas 
antioksidannya dengan metode DPPH (1,1-diphenyl-2-picrylhydrazyl). Protein 
hidrolisat dengan persentase inhibisi tertinggi ditentukan nilai IC50, AAI 
(Antioxidant Activity Index), aktivitas antiinflamasi, SDS-PAGE, dan uji hemolisis. 
Hasil penelitian menunjukkan aktivitas optimum enzim bekerja pada suhu 45 °C 
dan pH 8. Nilai derajat hidrolisis tertinggi tercapai pada waktu hidrolisis 60 menit 
sebesar 65,823%. Persentase inhibisi tertinggi terhadap radikal DPPH sebesar 
35,61% terjadi pada waktu inkubasi 10 menit dengan nilai IC50 sebesar 1,4364 
mg/mL dan AAI sebesar 0,013. Aktivitas antiinflamasi ditunjukkan dengan 
persentase inhibisi sebesar 26,61%. Keberadaan pita protein analisis SDS-PAGE 
yakni PIL pada 11; 35-48; dan 180 KDa, sementara PHL-10 pada <11 KDa. 
Persentase hemolisis diperoleh sebesar 4,93% yang menunjukkan bahwa protein 
hidrolisat ikan lele sedikit lisis terhadap sel darah merah.

Abtrak (Bhs. Inggris)

Degenerative diseases caused by free radicals have led to the development of 
natural antioxidant and anti-inflammatory compounds, one of which is bioactive 
peptides. Bioactive peptides can be obtained through enzymatic hydrolysis using 
catfish protein (Clarias gariepinus). The research stages included isolation of 
catfish protein, production of protease enzyme crude extract from Bacillus subtilis 
B209 bacteria, and characterization of temperature and pH. Catfish protein was 
hydrolysed using enzyme crude extract for various a times of 10, 20, 30, 40, 50, and 
60 minutes at optimum conditions and the degree of hydrolysis was determined and 
tested for antioxidant activity using the DPPH (1,1-diphenyl-2-picrylhydrazyl) 
method. Protein hydrolysate with the highest inhibition percentage were 
determined for IC50, AAI (Antioxidant Activity Index), anti-inflammatory activity, 
SDS-PAGE, and hemolysis test. The results showed that the optimum activity of the 
enzyme worked at a temperature of 45 °C and pH 8. The highest degree of 
hydrolysis was achieved at a hydrolysis time of 60 minutes at 65,823%. The highest 
percentage of inhibition against DPPH radicals of 35,61% occurred at an 
incubation time of 10 minutes with an IC50 value of 1,4364 mg/mL and AAI of 0,013. 
Anti-inflammatory activity shown with a percentage inhibition of 26.61%. The 
existance of protein bands in SDS-PAGE analysis, namely PIL at 11; 35-48; and 
180 KDa, while PHL-10 at <11 KDa. The percentage of hemolysis obtained was 
4,93%, indicating that catfish protein hydrolysate slightly lysed red blood cells.

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