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CHRISEPTIANA VENELIA

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Abstrak (Bhs. Indonesia)

Dendrobium lasianthera merupakan salah satu jenis anggrek endemik
Indonesia yang memiliki nilai ekonomi tinggi, tetapi populasinya di alam semakin
menurun akibat eksploitasi dan degradasi habitat. Penelitian ini bertujuan untuk
mengetahui pengaruh jenis media tanam dan warna cahaya LED terhadap
pertumbuhan D. lasianthera dalam kultur in vitro. Penelitian dilakukan
menggunakan Split-plot dengan kombinasi empat warna cahaya LED sebagai main
plot, yaitu C0 (putih), C1 (biru), C2 (merah), dan C3 (biru + merah), serta
kombinasi tiga jenis media tanam sebagai sub-plot, yaitu M0 (TBOK), M1
(Knudson C), dan M2 (½ vit + MS). Parameter yang diamati meliputi jumlah tunas,
jumlah akar, panjang akar, luas daun, dan jumlah daun. Hasil penelitian
menunjukkan bahwa media Knudson C (M1) memberikan pertumbuhan terbaik
pada jumlah tunas (1,27 tunas) dan luas daun (6,76 cm²), sedangkan media TBOK
(M0) menghasilkan panjang akar terbaik (2,32 cm). Warna cahaya LED putih (C0)
menghasilkan luas daun terbesar (5,25 cm²), sedangkan LED biru + merah (C3)
memberikan hasil terbaik pada jumlah tunas (0,97 tunas). Hasil uji lanjut Duncan’s
Multiple Range Test (DMRT) menunjukkan bahwa interaksi antara jenis media dan
warna cahaya LED berpengaruh nyata terhadap tinggi tanaman, panjang daun, luas
daun, dan jumlah akar. Kadar klorofil tertinggi terdapat pada kombinasi cahaya
biru+merah dibandingkan pada warna cahaya lain.

Abtrak (Bhs. Inggris)

Dendrobium lasianthera is a native Indonesian orchid with significant
economic importance, yet its natural population is decreasing as a result of
overexploitation and habitat destruction. This study aimed to determine the effect
of different culture media and LED light colors on the in vitro growth of D.
lasianthera. The research was conducted using a split-plot design with three types
of culture media: M0 (TBOK), M1 (Knudson C), and M2 (½ vit + MS), combined
with four LED light colors: C0 (white), C1 (blue), C2 (red), and C3 (blue + red).
The observed parameters included the number of shoots, number of roots, root
length, leaf area, and number of leaves. The results showed that Knudson C medium
(M1) produced the best growth in terms of the number of shoots (1.27 shoots) and
leaf area (6.76 cm²), while TBOK medium (M0) resulted in the longest root growth
(2.32 cm). White LED light (C0) produced the largest leaf area (5.25 cm²), while
blue + red LED light (C3) resulted in the highest number of shoots (0.97 shoots).
The Duncan’s Multiple Range Test (DMRT) indicated that the interaction between
culture media and LED light color had a significant effect on plant height, leaf
length, leaf area, and root number. The highest chlorophyll content was found in
the blue + red LED light combination compared to other light colors. This study
provides insights into optimizing the in vitro propagation of D. lasianthera to
support conservation efforts and sustainable orchid production.

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