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Abstrak (Bhs. Indonesia)

Agen biologis alternatif untuk pengendalian penyakit tanaman dibandingkan dengan pestisida sintetis. Bakteri potensial dalam pembuatan biopestisida formula cair adalah, B. subtillis NPA6. Optimalisasi biopestisida melalui analisis karakter kimia. Penelitian ini bertujuan untuk: 1. Mengarakter secara kimia formula cair sebagai pengendali hayati; 2. Mengetahui potensi bakteri endofit B. subtilis NPA6 sebagai agens pengendali hayati patogen Xanthomonas axonopodis bawang merah in vitro; 3. Mengetahui respon pertumbuhan bawang merah setelah diaplikasikan formula cair B. subtilis NPA6.
Penelitian dilakukan di Laboratorium Perlindungan Tanaman, Fakultas Pertanian, Universitas Jenderal Soedirman dan Pertanahan di Karangwangkal, Purwokerto Utara, pada bulan Juni hingga Desember 2024. Tahapan penelitian berupa, persiapan isolat dan suspensi B. subtilis NPA6, pembuatan biopestisida formula cair, pengujian karakter kimia formula, budidaya bawang merah dan aplikasi formula, pengamatan pertumbuhan, dan pengujian antagonis formula. Penelitian ini menggunakan Rancangan Acak Lengkap 3 perlakuan dan 8 ulangan yaitu: P0, P1, dan P2 di lapangan dan 4 perlakuan 5 ulangan: F0, F1, F2, dan F3 in vitro. Variabel pengamatan berupa karakter kimia, daya tahan B. subtilis NPA6, tinggi tanaman, jumlah daun, berat segar, berat kering umbi, panjang akar, dan ketahanan terhadap Xaa. 
Formula air kelapa mengandung nutrisi yang lebih tinggi dalam N dan Zn, air pencuci beras P, K, dan Cu. Parameter Fe dari rumus kedua adalah <0,003 mg/L. Isolat murni B. subtilis NPA6 memiliki mekanisme bakteriostatik kategori sedang. Formula air kelapa meningkatkan berat kering umbi sebesar 90% dan perlakuan formula air cuci beras sebesar 57% dibandingkan dengan kontrol. Kedua formula tersebut mampu meningkatkan jumlah daun.

Abtrak (Bhs. Inggris)

Alternative biological agents for plant disease control compared to synthetic pesticides. Potential bacteria in the manufacture of liquid formula biopesticides are, B. subtillis NPA6. Optimization of biopesticides through chemical character analysis. This research aims to: 1. Make and chemically characterize liquid formula as a biological controller; 2. To determine the potential of endophytic bacteria B. subtilis NPA6 as a biological control agent of the pathogen Xanthomonas axonopodis shallots in vitro; 3. To determine the growth response of shallots after applying the liquid formula of B. subtilis NPA6. The research was carried out at the Plant Protection Laboratory, Faculty of Agriculture, Jenderal Soedirman University and Land in Karangwangkal, North Purwokerto, from June to December 2024. Research stages, preparation of B. subtilis NPA6 isolate and suspension, manufacture of liquid formula biopesticides, testing of formula chemical character, cultivation of shallots and application of formula, observation of growth, and testing of formula antagonists. The study used a Complete Random Design of 3 treatments and 5 replicates, namely: P0, P1, and P2 in the field and 4 treatments of 3 replicates: F0, F1, F2, and F3 in vitro. Observation variables were in the form of the chemical character of the formula, the durability of B. subtilis NPA6 in the formula, plant height, number of leaves, fresh weight, dry weight of the tubers, root length, and resistance to Xaa. Coconut water formula contains higher nutrients in N and Zn, rice washing water P, K, and Cu. The Fe parameter of the second formula is <0.003 mg/L. Pure isolate of B. subtilis NPA6 has a medium category bacteriostatic mechanism. The coconut water formula increased the dry weight of the tubers by 90% and the rice washing water formula treatment by 57% compared to the controls. Both formulas are able to increase the number of leaves.

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