Update Artikelilmiah: 48051

SALWA FAISHAL AL-KATIRI

NIM

Judul Artikel

Abstrak (Bhs. Indonesia)

Genome sequencing menjadi tren baru untuk menganalisis klaster gen biosintesis (BGCs) dan mampu memprediksi produk metabolit sekunder yang dihasilkan. Sebanyak 19 dari 28 strain jamur Penicillium citrinum di database NCBI yang dianalisis dengan whole genome sequencing (WGS) berasal dari feses manusia. Penelitian ini bertujuan untuk menentukan tipe Biosynthetic Gene Clusters (BGCs), membandingkannya dengan strain jamur sejenis pada database NCBI, serta mengkaji potensi aktivitas predicted compounds yang ditemukan. Jamur P. citrinum K6A ditumbuhkan pada media PDA dan diinokulasi hingga diperoleh jamur tunggal dan murni. Analisis WGS dilakukan menggunakan Oxford Nanopore Technology (ONT). Tipe BGCs dari jamur ini dan 28 strain lainnya pada database NCBI dianalisis dengan antiSMASH dan BiG-SCAPE. Lebih lanjut, potensi antimikroba dari predicted compounds dikaji dari literatur. Hasil analisis antiSMASH didapatkan 12 contigs yang mengkode tipe BGCs, diantaranya NRPS-like yang terbanyak dan diperoleh 11 predicted compounds. Analisis BiG-SCAPE menemukan satu tipe BGC, yaitu NRPS unik yang hanya muncul pada strain jamur P. citrinum K6A. Berdasarkan kajian literatur, 6 dari 11 predicted compounds berpotensi sebagai antifungi, antibakteri, dan/atau antivirus, diantaranya equisetine yang memiliki ketiga aktivitas tersebut. Dengan demikian, P. citrinum K6A dapat dieksplorasi lebih lanjut melalui kajian transkriptomik untuk mengetahui ekspresi gen tersebut.

Abtrak (Bhs. Inggris)

Abstract
Genome sequencing has become a new trend in analyzing biosynthetic gene clusters (BGCs) and can predict the secondary metabolites of microorganisms. Nineteen of 28 strains of the Penicillium citrinum in the NCBI database analyzed with whole genome sequencing (WGS) originated from human feces. This study aims to determine the type of Biosynthetic Gene Clusters (BGCs), to compare them with similar fungal strains in the NCBI database, and to examine the potential activity of the predicted compounds. A fungus P. citrinum K6A was cultured on PDA media and inoculated until single and pure fungus was obtained. WGS analysis was performed using Oxford Nanopore Technology (ONT). The BGCs’s type from this fungus and 28 other strains in the NCBI database were analyzed using both antiSMASH and BiG-SCAPE. Furthermore, the antimicrobial potential of the predicted compounds was studied from the literature. The antiSMASH analysis revealed there were 12 contigs coding for 9 types of BGCs, for instance NRPS-like as the most frequent, and resulting in 11 predicted compounds. BiG-SCAPE analysis found one unique BGC’s type namely NRPS that only emerged in the P. citrinum K6A fungal strain. Based on the literature studied, 6 of 11 predicted compounds have the potential as antifungal, antibacterial, and/or antiviral, including equisetine which possessed all those activities. Thus, P. citrinum K6A can be further explored through transcriptomic studies to determine the expression of the gene.

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