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THE EFFECT OF VARIATIONS IN DRYING METHODS AND SOLVENT CONCENTRATION ON THE POLYPHENOL CONTENT OF STEVIA (Stevia rebaudiana Bertoni) LEAVES EXTRACT
Abstrak (Bhs. Indonesia)
Drying methods influence the stability and content of bioactive compounds in plant leaves, including polyphenols. While drying reduces water content, it may also cause degradation of bioactive compounds. Extraction methods and solvent concentration play crucial roles in determining polyphenol yield. Ethanol, a commonly used solvent, provides an optimal balance between water and alcohol polarity, facilitating effective phenolic compound extraction.This study aimed to investigate the effects of different drying methods and ethanol concentrations on the polyphenol content of Stevia leaf extract. The research was conducted experimentally, using a Completely Randomized Design (CRD) with a factorial treatment pattern. The drying method (oven drying, sun drying, and room temperature drying) and ethanol concentration (70% and 96%) were evaluated in six treatment combinations, each replicated three times, totaling 18 experimental units. Data were analyzed using ANOVA at a 5% significance level and correlation coefficient test. Results indicated that neither drying method nor solvent concentration had a significant effect on polyphenol content. Solvent concentration contributed 11.2% to the variation in polyphenol content, while the drying method contributed 2.6%. The highest polyphenol content 18.5 ± 16.5 mg GAE/g was obtained from oven drying with 70% ethanol extraction. The combination of sunlight drying method with 96% ethanol solvent produces the lowest total polyphenol content of 7.88 ± 6.11 mg GAE/g.
Abtrak (Bhs. Inggris)
Drying methods influence the stability and content of bioactive compounds in plant leaves, including polyphenols. While drying reduces water content, it may also cause degradation of bioactive compounds. Extraction methods and solvent concentration play crucial roles in determining polyphenol yield. Ethanol, a commonly used solvent, provides an optimal balance between water and alcohol polarity, facilitating effective phenolic compound extraction.This study aimed to investigate the effects of different drying methods and ethanol concentrations on the polyphenol content of Stevia leaf extract. The research was conducted experimentally, using a Completely Randomized Design (CRD) with a factorial treatment pattern. The drying method (oven drying, sun drying, and room temperature drying) and ethanol concentration (70% and 96%) were evaluated in six treatment combinations, each replicated three times, totaling 18 experimental units. Data were analyzed using ANOVA at a 5% significance level and correlation coefficient test. Results indicated that neither drying method nor solvent concentration had a significant effect on polyphenol content. Solvent concentration contributed 11.2% to the variation in polyphenol content, while the drying method contributed 2.6%. The highest polyphenol content 18.5 ± 16.5 mg GAE/g was obtained from oven drying with 70% ethanol extraction. The combination of sunlight drying method with 96% ethanol solvent produces the lowest total polyphenol content of 7.88 ± 6.11 mg GAE/g.
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