Update Artikelilmiah: 47643

PUSPITA TRI YULIANA

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Abstrak (Bhs. Indonesia)

Latar Belakang: Terapi sel punca mesenkimal (SPM) tali pusat manusia tengah
berkembang pesat. Namun, masih terdapat tantangan terapi seperti proliferasi dan migrasi
yang tidak terkendali. Sehingga, media terkondisi SPM sebagai upaya untuk meminimalisir
tantangan tersebut melalui sinkronisasi siklus sel sebelum transplantasi. Tujuan: Penelitian
ini bertujuan untuk mengetahui pengaruh starvasi terhadap kemampuan SPM tali pusat
manusia terhadap kemampuan migrasi dengan metode scratch assay. Metodologi: penelitian
eksperimental dengan pendekatan “post test only with control group” dengan sampel berupa
gambar area migrasi dari mikroskop inversi sebanyak 4 lapang pandang disetiap kelompok
perlakuan, yaitu kelompok media komplit pada DMEM+FBS (MK), starvasi serum pada
media DMEM only (BASAL) dan starvasi asam amino pada media HBSS (HBSS). Serta
pengambilan gambar menurut durasi starvasi. Perlakuan diulang sebanyak 1 kali.
Kuantifikasi luas penutupan migrasi SPM menggunakan software ImageJ. Analisis data
menggunakan One-Way ANOVA. Hasil: Terdapat perbedaan yang signifikan (p<0,05)
kemampuan migrasi SPM didalam 3 kelompok pada jam ke-4 dan 8. Terdapat perbedaan
yang signifikan (p<0,05) rerata kemampuan migrasi SPM pada media BASAL dan HBSS
disetiap periode durasi starvasi. Kesimpulan: Kemampuan migrasi pada kelompok HBSS
lebih lambat dibandingkan kelompok lainnya. Durasi starvasi terbaik pada tiga kelompok
media pada jam ke-4 dan 8.
Kata kunci: Migrasi, Sel punca mesenkimal, Siklus sel, starvasi

Abtrak (Bhs. Inggris)

Background: Human umbilical mesenchymal stem cell (hUMSC) therapy is rapidly
advancing. However, challenges persist in therapeutic applications, such as uncontrolled
proliferation and migration. Thus, conditioned media for stem cells has been explored as a
means to minimize these challenges by synchronizing the cell cycle prior to transplantation.
Objective: This study aims to investigate the effect of starvation on the migration ability of
human umbilical mesenchymal stem cells (hUMSCs) using the scratch assay method.
Methodology: An experimental study with a "post-test only with control group" approach
was conducted, with samples consisting of migration area images taken from an inverted
microscope at four different fields of view for each treatment group, namely the complete
media group in DMEM+FBS (MK), serum starvation in DMEM only (BASAL), andasam
amino starvation in HBSS media (HBSS). Images were captured according to the starvation
duration, and treatments were repeated once. Migration area quantification was performed
using ImageJ software. Data analysis was conducted using One-Way ANOVA. Results: A
significant difference (p<0.05) in migration ability was observed in the three groups at the
4th and 8th hours. A significant difference (p<0.05) in the average migration ability of
hUMSCs was noted in the BASAL and HBSS media across different starvation durations.
Conclusion: The migration ability in the HBSS group was slower compared to the other
groups. The optimal starvation duration across all media groups was observed at the 4th
and 8th hours.
Keywords: Cell cycle, Mesenchymal stem cells, Migration, Starvation

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