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ISTI FAUROH

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Abstrak (Bhs. Indonesia)

Channa asiatica merupakan ikan gabus hias berasal dari China yang hidup di air tawar dan termasuk ikan karnivora. Pakan yang dikonsumi didigesti di sepanjang saluran pencernaan yang melibatkan aktivitas enzim, yaitu protease dan amilase.  Aktivitas protease dan amilase dipengaruhi oleh beberapa faktor diantaranya ukuran tubuh dan organ digesti yang berbeda. Tujuan dari penelitian ini adalah untuk mengetahui adanya perbedaan aktivitas protease dan amilase ikan C. asiatica antar ukuran tubuh, antar organ digesti dan interaksi antara ukuran tubuh dengan organ digesti dan mengetahui aktivitas protease dan amilase yang lebih tinggi di antara ukuran tubuh dan organ digesti berbeda pada ikan C. asiatica. Metode dalam penelitian ini menggunakan eksperimental dengan rancangan percobaan Rancangan Acak Lengkap pola faktorial 3x4 Data dianalisis dengan two-ways ANOVA dan analisis lanjut Duncan. Hasil penelitian aktivitas protease C. asiatica menunjukkan perbedaan antar ukuran tubuh, organ digesti, maupun interaksi keduanya (P<0,05). Semakin besar ukuran tubuh ikan, maka akan semakin tinggi aktivitas proteasenya. Aktivitas protease di pilorik kaeka dan usus lebih tinggi daripada di hepatopankreas dan lambung. Hasil interaksi ukuran tubuh dan organ digesti juga menunjukkan bahwa pilorik kaeka dan usus ikan ukuran 100 g memiliki aktivitas protease tertinggi. Aktivitas amilase tidak berbeda secara signifikan antar ukuran tubuh (P>0,05), namun antar organ digesti maupun interaksi keduanya ada perbedaan (P<0,05). Aktivitas amilase di lambung dan usus lebih tinggi daripada organ digesti lainnya. Hasil interaksi ukuran tubuh dan organ digesti yang menunjukkan bahwa lambung ikan berukuran 2,5 g memiliki aktivitas amilase tertinggi.

Abtrak (Bhs. Inggris)

Channa asiatica, a decorative snakehead fish native to China, inhabits freshwater environments and is carnivorous. The food consumed by this species is digested along the digestive tract through enzymatic activities involving protease and amylase. The activities of these enzymes are influenced by factors such as body size and variations in digestive organs. This study aimed to assess differences in protease and amylase activities in C. asiatica across various body sizes, digestive organs, and the interaction between body size and digestive organs. Additionally, it aimed to determine the enzyme activity that is highest across different body sizes and digestive organs in C. asiatica.The study employed an experimental method using a Completely Randomized Design (CRD) with a 3x4 factorial design. Data were analyzed using two-way ANOVA followed by Duncan's post hoc test. The results showed that protease activity in C. asiatica varied significantly with body size, digestive organ, and their interaction (P<0.05). Larger body sizes were associated with higher protease activity. Protease activity was higher in the pyloric caeca and intestines compared to the hepatopancreas and stomach. Interaction between body size and digestive organ further indicated that the pyloric caeca and intestines of fish weighing 100 g exhibited the highest protease activity. Amylase activity did not differ significantly across body sizes (P>0.05); however, there were significant differences across digestive organs and in the interaction between body size and organ (P<0.05). Amylase activity was higher in the stomach and intestines compared to other digestive organs, with the interaction showing that the stomach of fish weighing 2.5 g had the highest amylase activity.

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