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WIRANTI MAWARNI

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Abstrak (Bhs. Indonesia)

Penelitian ini dilaksanakan di laboratorium perlindungan tanaman dan screen house Fakultas Pertanian, Universitas Jenderal Soedirman, Karangwangkal, Purwokerto Utara. Penelitian ini dilaksanakan pada Desember 2023 hingga Mei 2024. Penelitian dilakukan secara in vitro dan in planta. Penelitian in vitro dilakukan dengan Rancangan Acak Lengkap (RAL) 2 faktor, yaitu konsentrasi ekstrak meniran dan pelarut. Konsentrasi yang digunakan terdiri dari 6 taraf, yaitu 0%, 2%, 4%, 6%, 8%, dan 10%. Pelarut yang digunakan yaitu air dan etanol 96%. Adapun penelitian secara in planta dilakukan dengan Rancangan Acak Kelompok (RAK) non faktorial dengan 4 perlakuan, yaitu P0 = kontrol/tanpa perlakuan, P1= bakterisida (bahan aktif tembaga oksida 56%), P2 = ekstrak meniran 10% pelarut air, dan P3 = ekstrak meniran 10% pelarut etanol 96%. Hasil penelitian menunjukkan bahwa ekstrak meniran mengandung senyawa antibakteri dan mampu menghambat pertumbuhan bakteri Xoo secara in vitro. Ekstrak dengan konsentrasi 10% pada pelarut etanol 96% menghasilkan zona hambat paling baik. Namun, Aplikasi ekstrak meniran 10% dengan pelarut air maupun etanol 96% kurang efektif dalam menekan penyakit hawar daun bakteri (HDB) pada tanaman ditinjau dari variabel periode inkubasi, intensitas penyakit, dan laju infeksi yang tidak berbeda nyata pada setiap perlakuan. Aplikasi ekstrak meniran 10% dengan pelarut air maupun etanol 96% juga tidak memberikan berpengaruh terhadap pertumbuhan dan hasil tanaman padi.

Abtrak (Bhs. Inggris)

This research was carried out at the plant protection laboratory and screen house Faculty of Agriculture, Jenderal Soedirman University, Karangwangkal, North Purwokerto. This research was carried out from December 2023 to May 2024. The research was conducted in vitro and in plant. In vitro research was conducted with a 2-factor Completely Randomized Design (CRD), namely the concentration of P. niruri extract and solvent. The concentration used consists of 6 levels, namely 0%, 2%, 4%, 6%, 8%, and 10%. The solvents used are water and ethanol 96%. The study in plant was carried out with a non-factorial Randomized Complete Block Design (RCBD) with 4 treatments, namely P0 = control, P1 = bactericide (active ingredient copper oxide 56%), P2 = 10% extract with water solvent, and P3 = 10% extract with 96% ethanol solvent. The results showed that P. niruri extract contained antibacterial compounds and was able to inhibit the growth of Xoo bacteria in vitro. Extracts with a concentration of 10% in ethanol solvents of 96% produce the best inhibition zone. However, the application of 10% P. niruri extract with water solvent and 96% ethanol was less effective in suppressing bacterial leaf blight (BLB) in plants from the variables of incubation period, disease intensity, and infection rate which did not differ significantly in each treatment. The application of 10% P. niruri extract with water solvent or 96% ethanol also has no effect on the growth and yield of rice plants.

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