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ANISA NURUL ISNAENI

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Abstrak (Bhs. Indonesia)

Ikan gelodok (Boleophthalmus boddarti) merupakan ikan yang sangat mudah dijumpai di wilayah hutan mangrove dan mengandung protein tinggi. Proses peningkatan kadar protein ikan gelodok dapat dilakukan dengan perendaman natrium bikarbonat (NaHCO3) pada saat sebelum pengolahan tepung ikan gelodok. Kemudian dilanjutkan hidrolisis secara enzimatis untuk menghasilkan peptida aktif yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui kadar protein, persentase derajat hidrolisis, serta aktivitas antibakteri dari hidrolisat protein ikan gelodok dengan dan tanpa perendaman NaHCO3 serta pengaruh perbedaan konsentrasi terhadap bakteri Vibrio parahaemolyticus. Penelitian ini dilakukan metode eksperimental laboratorium melalui tiga tahapan, yaitu 1) preparasi sampel, 2) hidrolisis protein, pengukuran derajat hidrolisis dan kadar protein, 3) uji aktivitas antibakteri dengan konsentrasi yang berbeda (100, 150, 200, dan 250 mg/mL). Hasil penelitian menunjukkan bahwa kadar protein perlakuan perendaman NaHCO3  lebih tinggi dibandingkan tanpa perendaman NaHCO3 dengan nilai masing-masing sebesar 83,65±0,09% dan 80,48±0,30. Hasil yang sama juga diperoleh pada derajat hidrolisis perlakuan perendaman NaHCO3  lebih tinggi dibandingkan tanpa perendaman NaHCO3 dengan nilai masing-masing sebesar 81,58±3,64% dan 17,83±1,32%. Hasil aktivitas antibakteri hidrolisat dari kedua perlakuan relatif lemah yakni hanya berkisar antara 0,74±0,17 mm hingga 1,61±0,02 mm, namun perbedaan konsentrasi ekstrak hidrolisat protein menunjukkan pengaruh terhadap luas zona hambat bakteri.

Abtrak (Bhs. Inggris)

The mudskipper (Boleophthalmus boddarti) is a fish commonly found in mangrove area and it has high protein content. The protein content of mudskipper fish can be enhanced through soaking in sodium bicarbonate (NaHCO₃) before processing it into fish flour. This is followed by enzymatic hydrolysis to produce active peptides with potential antibacterial properties. This study aims to determine the protein content, degree of hydrolysis, and antibacterial activity of mudskipper protein hydrolysates with and without NaHCO₃ soaking, as well as the effect of different concentrations on Vibrio parahaemolyticus. The study was conducted using a laboratory experimental method through three stages: 1) sample preparation, 2) protein hydrolysis, measurement of the degree of hydrolysis and protein content, and 3) antibacterial activity testing with different concentrations (100, 150, 200, and 250 mg/mL). The results showed that the protein content of the NaHCO₃ soaked treatment was higher than that of the treatment without NaHCO₃ soaking, with respective values of 83.65±0.09% and 80.48±0.30%. Similarly, the degree of hydrolysis was higher in the NaHCO₃ soaked treatment compared to the treatment without NaHCO₃ soaking, with respective values of 81.58±3.64% and 17.83±1.32%. The results showed that the antibacterial activity of the hydrolysates from both treatments was relatively weak, ranging only from 0.74±0.17 mm to 1.61±0.02 mm, the difference in hydrolysate protein extract concentration showed an effect on the size of the bacterial inhibition zone.

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