Update Artikelilmiah: 45538

LESTHA AUREL SALSABILLA

NIM

Judul Artikel

Abstrak (Bhs. Indonesia)

Peralatan laboratorium yang terjangkau sangat penting untuk 
pengembangan dan pemanfaatan ilmu secara berkelanjutan. Penelitian ini 
menyajikan pengembangan spektrofotometer sederhana yang terjangkau untuk 
mengukur kadar protein dengan metode Lowry. Penelitian ini bertujuan untuk mengetahui potensi dan validasi spektrofotometer sederhana menggunakan LED, 
sensor cahaya dan mikrokontroler untuk penentuan kadar protein dengan metode 
Lowry. Perancangan spektrofotometer ini menggunakan komponen 
mikrokontroler Arduino, sensor cahaya TSL2591, LED 750 nm sebagai sumber 
cahaya, rumah komponen dan tempat kuvet dirancang dengan software 3D dan 
dicetak dengan printer cetak 3D menggunakan filament PLA (Polylactic Acid). 
Spektrofotometer selanjutnya diuji dan divalidasi meliputi kinerja alat, uji validasi (linearitas, LOD dan LOQ, rentang metode, presisi, presisi antara, akurasi), dan 
penentuan kadar protein dalam sampel susu kambing. Hasil pengujian dengan spektrofotometer sederhana menunjukkan rentang linear 100 – 700 ppm, dengan 
nilai koefisien korelasi (r) 0,9995; batas deteksi (LOD) 56,751 ppm; nilai batas kuantifikasi 189,170 ppm; nilai batas bawah (LOQ) 189,170 ppm dan nilai batas 
atas 734,100 ppm; presisi dengan nilai HORRAT 0,831; presisi antara dengan 
nilai HORRAT 0,820; akurasi dengan nilai rata-rata persen perolehan kembali 
(%Recovery) 102%; kadar protein dalam sampel susu kambing 415,100 ppm.

Abtrak (Bhs. Inggris)

Affordable laboratory equipment is essential for the sustainable 
development and utilization of science. This research presents the development of 
a simple, affordable spectrophotometer to measure protein levels using the Lowry 
method. The research aims to determine the potential and validate a simple spectrophotometer using LEDs, light sensors, and microcontrollers for 
determining protein levels by the Lowry method. The design of this 
spectrophotometer incorporates Arduino microcontroller components, a TSL2591 
light sensor, a 750 nm LED as a light source, component housing, and a cuvette 
holder designed with 3D software and printed with a 3D printer using PLA 
(Polylactic Acid) filament. The spectrophotometer was then tested and validated, 
including tool performance and validation tests (linearity, LOD, and LOQ, method 
range, precision, intermediate precision, accuracy), as well as the determination of 
protein levels in goat milk samples. The test results with the simple 
spectrophotometer showed a linear range of 100 - 700 ppm, with a correlation 
coefficient (r) value of 0.9995; a limit of detection (LOD) of 56.751 ppm; a limit 
of quantification (LOQ) of 189.170 ppm; a lower limit value of 189.170 ppm and 
an upper limit value of 734.100 ppm; precision with a HORRAT value of 0.831; 
intermediate precision with a HORRAT value of 0.820; and accuracy with an 
average percent recovery value of 102%. The protein content in goat milk samples 
was determined to be 415.100 ppm.

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