Update Artikelilmiah: 44685

KHARISMA AULIA CINTANSIH

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Abstrak (Bhs. Indonesia)

Nudibranchia dan jamur endofit dilaporkan berpotensi sebagai senyawa antimikroba. Namun, kultur jamur endofit dalam kondisi laboratorium standar sering gagal akibat klaster gen biosintesis yang tidak aktif. Penerapan strategi One Strain MAny Compounds (OSMAC) menjadi penyelesaian permasalahan tersebut. Penelitian ini bertujuan untuk mengisolasi jamur endofit simbion Nudibranchia dan mengidentifikasi metabolit serta menguji aktivitas antibakterinya. Isolat jamur endofit kode 8 simbion Nudibrancia murni dikultivasi pada media beras dengan penambahan garam krosok sebagai penerapan OSMAC dan media beras tanpa garam krosok (non OSMAC). Jamur diekstraksi dengan etil asetat dan difraksinasi dengan metanol:n-heksana lalu diuji KLT dan aktivitas antibakteri terhadap Mycobacterium smegmatis dengan metode difusi cakram. Berdasarkan profil KLT, ekstrak EtOAc dan fraksi MeOH jamur endofit simbion Nudibranchia hasil OSMAC mengandung alkaloid, flavonoid, polifenol dan steroid yang berbeda dengan kultur non OSMAC yang hanya mengandung flavonoid, polifenol dan steroid. Fraksi MeOH OSMAC menunjukkan aktivitas penghambatan terhadap M. smegmatis dengan diameter zona hambat sebesar 0,5 mm (lemah). Dengan demikian, jamur endofit simbion Nudibranchia kode 8 yang dikultur dengan metode OSMAC pada media beras dengan penambahan garam krosok menginduksi metabolit sekunder yang lebih beragam dan berpotensi sebagai antibakteri terhadap M. smegmatis.

Abtrak (Bhs. Inggris)

Nudibranchia and endophytic fungi were reported to have potential as antimicrobial. Nevertheless, endophytic fungi cultures under standard laboratory conditions often fail due to inactive biosyntetic gene clusters. The application of the One Strain MAny Compounds (OSMAC) strategy is a solution to this problem. This study aims to isolate the endophytic fungi associated with Nudibranchia, to identify fungal metabolites and to evaluate their antibacterial activity. Pure isolate of Endophytic fungi associated with Nudibranchia code 8 were cultivated on rice media with the addition of coarse salt as an application of OSMAC and on rice media without coarse salt (non OSMAC). The endophytic fungi were extracted with ethyl acetate and fractionated with methanol:n-hexane then analyzed by TLC and tested for antibacterial activity against Mycobacterium smegmatis using the disk diffusion method. Based on the TLC profile, the EtOAc extract and MeOH fraction of the endophytic fungi associated with Nudibranchia resulting from OSMAC contained alkaloid, flavonoid, polyphenol and steroid which had different compared to cultured with non-OSMAC which only contained flavonoid, polyphenol and steroid. The MeOH OSMAC fraction showed inhibitory activity against M. smegmatis with an inhibitory zone diameter of 0,5 mm (weak). Thus, the endophytic fungi associated with Nudibranchia code 8 cultured using the OSMAC method on rice media with the addition of coarse salt induced more diverse secondary metabolites and has potential as an antibacterial against M. smegmatis.

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