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ISMIRALDA OKE PUTRANTI

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Abstrak (Bhs. Indonesia)

Masalah. Studi ini dilakukan untuk menemukan sifat antioksidan dan perannya sebagai fotoprotektor dan agen anti-inflamasi pada model tikus sunburn. Pendekatan. Biokimia dengan spektrofotometer dilakukan untuk melacak kandungan flavonoid, fenol, saponin, tanin, steroid (kualitatif). Lendir siput dibagi menjadi 4 konsentrasi 20%, 50%, 70%, dan 100% yang diterapkan pada punggung tikus 1 jam sebelum radiasi UV. Studi eksperimental ini dengan desain kelompok kontrol hanya tes dilakukan untuk mempelajari peran fotoprotektor dan anti-inflamasi dengan menggunakan 75 Mus musculus BALB/c tikus model sunburn yang dibagi menjadi 15 grup. Efek fotoprotektor diukur secara semi-kuantitatif dari perubahan histopatologis 24 jam setelah radiasi, sementara efek anti-inflamasi ditentukan dengan menggunakan imunohistokimia anti-Interleukin-6 (IL-6) dan hitungan leukosit darah perifer. Temuan. Ekstrak lendir siput A. fulica terbukti mengandung flavonoid (+++), fenol (++), saponin (+++), dan steroid (++). Aplikasi lendir siput sebelum radiasi UV secara signifikan (p<0,000) mencegah perubahan histopatologis kulit dalam efek sunburn dari konsentrasi terendah 20%, terbukti mencegah pembentukan sel sunburn, pembentukan kerak, ulserasi, dan inflamasi dermal. Hasil imunohistokimia anti-IL-6 juga menunjukkan bahwa lendir siput juga mencegah pelepasan IL-6 dalam sunburn secara signifikan (p<0,01) dan menurunkan jumlah leukosit darah perifer (p<0,000). Kesimpulan. Lendir siput A. fulica mengandung sifat antioksidan seperti flavonoid, fenol, saponin, dan steroid, dan berpotensi berperan sebagai fotoprotektor dan agen anti-inflamasi dalam sunburn.

Abtrak (Bhs. Inggris)

Problem. This study was conducted to find the antioxidant properties and its roles as photoprotector
and anti-inflammatory agent in sunburn model mice. Approach. Biochemistry with
spectrophotometer was conducted to trace the content of flavonoid, phenols, saponin, tannin, steroid
(qualitative). The snail slime was divided into 4 concentration 20%, 50%, 70% and 100% that were
applied onto the back of the mice 1hour prior UV radiation. This experimental study with test only
control group design was conducted to study photoprotector and anti-inflammatory role a by using 75
Mus musculus BALB/c sunburn model mice were divided into 15 groups. The photoprotector effect was
measured semi-quantitatively from the histopathologic changes 24 hours after radiation, meanwhile
the anti-inflammatory effect was determined by using immunohistochemistry of anti-Interleukin-6 (IL-
6) and leukocytes count of peripheral blood. Findings. The A. fulica snail slime extract was proven to
contain flavonoid (+++), phenols (++), saponin (+++) and steroid (++). Snail slime application prior
UV radiation showed significantly (p<0,000) prevent the histopathologic changes of the skin in
sunburn effect from the lowest concentration 20%, it showed to prevent sunburn cell formation, crust
formation, ulceration and dermal inflammation. The result of immunohistochemistry of anti-IL-6 also
showed the snail slime also prevented the release of IL-6 in sunburn significantly (p<0,01) and
decreased the leukocyte count of peripheral blood (p<0,000). Conclusion. The A.fulica snail slime
contained antioxidant properties such as flavonoid, phenols, saponin and steroid, and potentially
played role as photoprotector and anti-inflammatory agent in sunburn.

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