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IKEGWU CHUKWUDI MICHAEL
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Sea Turtle Species in Turtle Bay of Cilacap, Indonesia Revealed by DNA Barcoding Technique
Abstrak (Bhs. Indonesia)
DNA barcoding is an invaluable tool for swiftly and accurately identifying marine turtle species, especially when faced with challenges in morphological identification. This study aimed to address the limited data on the diversity of turtle species in Turtle Bay, Cilacap, Indonesia. The research employed morphological identification through meticulous observations and precise measurements of parameters (carapace length, clutch size, plastron color, and track width) and mtDNA barcoding for species identification. Four specific primers (VF2_t1, FishF2_t1, FishR2_t1, FR1d_t1) amplified two tissues and two embryos from different individuals, showing 100% similarity and identity with sequences in BOLDsystems and GenBank. Among the four known sea turtle species in Turtle Bay of Cilacap (Lepidochelys olivacea, Chelonia mydas, Eretmochelys imbricata, and Dermochelys coriacea), the study identified L. Olivacea (3 samples) and E. imbricata (1 sample). L. olivacea dominated the study area, and the absence of nesting Hawksbill sea turtles (E. imbricata) prompted the analysis of tissues from a deceased turtle found offshore. This data significantly contributes to ongoing marine turtle conservation efforts. Further research is imperative to identify the two additional species, validating the existence of four turtle species in Turtle Bay of Cilacap, Indonesia.
Abtrak (Bhs. Inggris)
DNA barcoding is an invaluable tool for swiftly and accurately identifying marine turtle species, especially when faced with challenges in morphological identification. This study aimed to address the limited data on the diversity of turtle species in Turtle Bay, Cilacap, Indonesia. The research employed morphological identification through meticulous observations and precise measurements of parameters (carapace length, clutch size, plastron color, and track width) and mtDNA barcoding for species identification. Four specific primers (VF2_t1, FishF2_t1, FishR2_t1, FR1d_t1) amplified two tissues and two embryos from different individuals, showing 100% similarity and identity with sequences in BOLDsystems and GenBank. Among the four known sea turtle species in Turtle Bay of Cilacap (Lepidochelys olivacea, Chelonia mydas, Eretmochelys imbricata, and Dermochelys coriacea), the study identified L. Olivacea (3 samples) and E. imbricata (1 sample). L. olivacea dominated the study area, and the absence of nesting Hawksbill sea turtles (E. imbricata) prompted the analysis of tissues from a deceased turtle found offshore. This data significantly contributes to ongoing marine turtle conservation efforts. Further research is imperative to identify the two additional species, validating the existence of four turtle species in Turtle Bay of Cilacap, Indonesia.
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