Update Artikelilmiah: 43344

ALDILA USWA PRASTIKA

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Abstrak (Bhs. Indonesia)

Latar Belakang. Penelitian dengan judul penambahan sari buah tomat dalam pengencer semen dengan lama penyimpanan yang berbeda terhadap motilitas dan viabilitas spermatozoa ayam pelung bertujuan untuk mengamati persentase motilitas dan viabilitas spermatozoa ayam pelung. Penelitian dilakukan di Experimental Farm dan Laboratorium Produksi Ternak Unggas, Fakultas Peternakan, Universitas Jenderal Soedirman, Purwokerto. Materi dan Metode. penelitian yang digunakan adalah 3 ekor ayam pelung jantan. Bahan yang digunakan yaitu semen ayam pelung, tomat, kuning telur, SDS, ringer laktat, trisodium sitrat dihidrat dan larutan eosin nigrosin. Alat yang digunakan yaitu mikroskop, mikrotube, mikropipet, mikrotip, timbangan analitik, centifunge, centifunge tube, spuit, object glass, cover glass, egg sparator, blender, kertas saring Whatman, gelas ukur, saringan dan kulkas. Metode penelitian menggunakan rancangan split plot dengan 4 perlakuan dan 5 kali ulangan. Penambahan sari buah tomat (0%, 9%, 17%, 23%) sebagai main plot, sementara lama penyimpanan (0, 2, 4, 6, 8, 10 jam) sebagai sub plot. Variabel yang diamati meliputi motilitas dan viabilitas spermatozoa ayam pelung, dan analisis data dilakukan dengan uji lanjut Duncant Multiple Range Test (DMRT). Hasil. Hasil analisis menunjukkan bahwa penambahan sari buah tomat berpengaruh nyata (P<0,05) terhadap motilitas dan viabilitas spermatozoa ayam pelung, sementara lama penyimpanan berpengaruh sangat nyata (P<0,01) terhadap motilitas spermatozoa ayam pelung. Simpulan. penambahan sari buah tomat sebesar 9% dalam pengencer menghasilkan persentase motilitas dan viabilitas yang paling optimal. Selain itu, lama penyimpanan selama 10 jam masih menghasilkan persentase motilitas dan viabilitas yang layak untuk digunakan inseminasi buatan.

Abtrak (Bhs. Inggris)

Background. A study titled "The Addition of Tomato Juice in Semen Extender with Different Storage Times on the Motility and Viability of Pelung Rooster Spermatozoa" aimed to observe the percentage of motility and viability of pelung rooster spermatozoa. The research was conducted at the Experimental Farm and Poultry Production Laboratory, Faculty of Animal Husbandry, Universitas Jenderal Soedirman, Purwokerto. Materrials and Methods. The study utilized 3 pelung roosters. The materials used included pelung rooster semen, tomato, egg yolk, SDS, lactate ringer, trisodium citrate dihydrate, and eosin nigrosin solution. The equipment used comprised a microscope, microtubes, micropipettes, microtips, analytical balance, centrifuge, centrifuge tubes, syringe, object glass, cover glass, egg separator, blender, Whatman filter paper, measuring glass, sieve, and refrigerator. The research method employed a split-plot design with 4 treatments and 5 replications. The addition of tomato juice (0%, 9%, 17%, 23%) served as the main plot, while the storage time (0, 2, 4, 6, 8, 10 hours) served as the subplot. The observed variables included the motility and viability of pelung rooster spermatozoa, and data analysis was conducted using the Duncan's Multiple Range Test (DMRT). Results. The analysis results indicated that the addition of tomato fruit extract significantly influenced (P<0.05) the motility and viability of pelung rooster spermatozoa, while the storage time significantly affected (P<0.01) the motility of Pelung chicken spermatozoa. Conclusion. The addition of 9% tomato juice in the extender resulted in the most optimal percentage of motility and viability. Additionally, a storage time of 10 hours still produced a viable percentage of motility and viability suitable for artificial insemination.

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