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The Growth and Protein Content of Microalgae Porphyridium cruentum on Culture Media with Various Salinity
Abstrak (Bhs. Indonesia)
Porphyridium cruentum is a type of microalgae from the Rhodophyceae group which can meet the needs of natural feed and other needa such as biofuel production, cosmetics, pharmaceuticals, and food additives. Microalgae can be cultivated in certain environmental conditions to grow optimally, one of which is the level of salinity. The purposes of this research are to study the growth and biomass of Porphyridium cruentum, to determine the production of biomass and protein content of Porphyridium cruentum cultured in the media with various concentration of salinity, and to determine the best salinity concentration of media culture to growth and produce biomass and protein of Porphyridium cruentum. The research applied an experimental method with Completely Randomized Design (CRD). Porphyridium cruentum was cultured at 6 different concentrations of salinity namely 0, 5, 10, 15, 20, and 25 ppt. the cell density of each salinity is observed until it reaches the death phase. Cell density data was analyzed quantitatively using Analysis of Variance (ANOVA) with a confidence level of 95% and 99% using SPSS. The results using the ANOVA test showed a very significant effect of various salinities on culture media on cell density each day. Further tests were carried out using the LSD test, and the results show the best salinity with the highest cell density is 25 ppt with average of 91,154 cells/mL, while the lowest cell density is 0 ppt with average of 14,854 cells/mL. Biomass increases effectively at salinity 25 ppt, and so does the protein content. The highest protein content obtained from salinity treatment was 25 ppt.
Abtrak (Bhs. Inggris)
Porphyridium cruentum is a type of microalgae from the Rhodophyceae group which can meet the needs of natural feed and other needa such as biofuel production, cosmetics, pharmaceuticals, and food additives. Microalgae can be cultivated in certain environmental conditions to grow optimally, one of which is the level of salinity. The purposes of this research are to study the growth and biomass of Porphyridium cruentum, to determine the production of biomass and protein content of Porphyridium cruentum cultured in the media with various concentration of salinity, and to determine the best salinity concentration of media culture to growth and produce biomass and protein of Porphyridium cruentum. The research applied an experimental method with Completely Randomized Design (CRD). Porphyridium cruentum was cultured at 6 different concentrations of salinity namely 0, 5, 10, 15, 20, and 25 ppt. the cell density of each salinity is observed until it reaches the death phase. Cell density data was analyzed quantitatively using Analysis of Variance (ANOVA) with a confidence level of 95% and 99% using SPSS. The results using the ANOVA test showed a very significant effect of various salinities on culture media on cell density each day. Further tests were carried out using the LSD test, and the results show the best salinity with the highest cell density is 25 ppt with average of 91,154 cells/mL, while the lowest cell density is 0 ppt with average of 14,854 cells/mL. Biomass increases effectively at salinity 25 ppt, and so does the protein content. The highest protein content obtained from salinity treatment was 25 ppt.
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