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Abstrak (Bhs. Indonesia)

Suplementasi garam dapat meningkatkan kadar garam di dalam tubuh, dan
mempengaruhi tingkat stres ikan. Uji salinitas digunakan sebagai uji stres pada 
ikan. Tujuan penelitian ini yaitu mengetahui profil darah dan tingkah laku ikan 
Nilem (Osteochilus vittatus) yang diberi suplementasi garam pada uji stres 
salinitas meliputi kadar glukosa dalam darah, kadar hematokrit, kadar 
hemoglobin, pergerakan ikan, bukaan operkulum, dan tingkah laku ikan. 
Metode penelitian ini adalah metode eksperimental. Penelitian ini menggunakan 
Rancangan Acak Lengkap (RAL) dengan 3 kali ulangan. Ikan Nilem yang diberi 
pakan dengan suplementasi garam selama 60 hari dengan dosis 
(0%,1%,2%,3%,dan 4%) dilakukan uji stres pada salinitas 10 ppt selama 10 menit
sebelum pengamatan parameter darah ikan. Hasil analisis data menunjukkan 
bahwa suplementasi garam pada pakan berpengaruh nyata (P<0,05) terhadap 
kadar hemoglobin dan kadar hematokrit relatif pada ikan Nilem setelah uji stres 
salinitas. Kadar hemoglobin dan hematokrit relatif tertinggi (122%) terdapat 
pada perlakuan dengan suplementasi garam 0%, sementara kadar hemoglobin
relatif terendah (93%) terdapat pada perlakuan dengan suplementasi garam 3%.
Pergerakan ikan Nilem tanpa suplementasi garam selama uji stres salinitas 10 
ppt cenderung diam di dasar tempat pemeliharaan. Sedangkan pergerakan ikan 
Nilem dengan pemberian suplementasi garam selama uji stres salinitas 
cenderung lebih aktif dan berenang di dasar tempat pemeliharaan.

Abtrak (Bhs. Inggris)

Salt supplementation can increase salt levels in the body, and affect fish stress 
levels. The salinity test was used as a stress test on fish. The aim of this research 
was to determine the blood profile and behavioral behavior of Nilem fish 
(Osteochilus vittatus) which were given salt supplementation in the salinity 
stress test including blood glucose levels, hematocrit levels, hemoglobin levels, 
fish movement, operculum termination, and fish behavioral behavior. This 
research method was an experimental method. This research used a Completely 
Randomized Design (CRD) with 3 replications. Nilem fish that were fed with salt 
supplementation for 60 days at doses (0%, 1%, 2%, 3%, and 4%) were subjected 
to a stress test at a salinity of 10 ppt for 10 minutes before observing the fish's 
blood parameters. The results of data analysis showed that salt supplementation 
in feed had a significant effect (P<0.05) on hemoglobin levels and relative 
hematocrit levels in Nilem fish after the salinity stress test. The highest relative 
hemoglobin and hematocrit levels (122%) were found in the treatment with 0% 
salt supplementation, while the lowest relative hemoglobin levels (93%) were 
found in the treatment with 3% salt supplementation. The movement of Nilem 
fish without salt supplementation during the 10 ppt salinity stress test tended to 
remain still at the bottom of the rearing area. Meanwhile, the movement of Nilem 
fish with salt supplementation during the salinity stress test tended to be more 
active and swam at the bottom of the rearing area.

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