Update Artikelilmiah: 42191

ANNISA RAHMAH

NIM

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Abstrak (Bhs. Indonesia)

Stabilitas kloramfenikol dipengaruhi oleh hidrolisis ikatan amida di bawah kondisi asam, basa, termal. Analisis hasil degradasi paksa dengan instrumen sederhana dan akurat seperti spektrofotometri derivatif dan kemometrik diterapkan pada penelitian ini. Uji ini untuk melihat apakah spektrofotometri dapat menunjukkan degradasi berdasarkan persentase degradasi. Uji linearitas, akurasi, dan presisi dilakukan dengan larutan standar 4-16 µg/mL. Uji degradasi paksa dilakukan dengan menempatkan sampel pada kondisi asam (HCl 0,1 N; 80oC selama 2 jam), basa (NaOH 0,1 N; 80oC selama 2 jam), dan termal (90oC selama 4 jam) lalu diukur dengan spektrofotometri derivatif. Analisis kemometrik dilakukan dengan PCA dan PLS-DA. Panjang gelombang maksimum kloramfenikol berurutan dari derivat ke nol hingga ketiga yaitu 278 nm, 260 nm, 234 nm, 292 nm. Metode memiliki linearitas yang baik (0,995 < r < 1). Kloramfenikol cenderung terdegradasi kecil dalam asam dan besar dalam basa maupun termal. Analisis kemometrik menunjukkan sampel asam cenderung mengelompok dekat kontrol. Spektrofotometri derivatif dapat menunjukkan persentase degradasi kloramfenikol walaupun tidak dapat dipastikan terkait produk degradasinya. Pada analisis kemometrik diperoleh pengaruh degradasi berdasarkan pengelompokan sampel kontrol dan perlakuan.

Abtrak (Bhs. Inggris)

The stability of chloramphenicol is influenced by the hydrolysis of amide bond under acidic, alkaline, thermal conditions. Analysis of forced degradation results with simple and accurate instruments such as derivative spectrophotometry and chemometrics was applied in this research. This study aims to see whether spectrophotometry can show degradation based on percentage of degradation. Linearity, accuracy and precision tests were carried out with 4-16 µg/mL of standard solutions. The forced degradation study was carried out by placing the sample in acidic (0.1 N HCl; 80oC for 2 hours), alkaline (0.1 N NaOH; 80oC for 2 hours), and thermal (90oC for 4 hours) then measuring it using derivative spectrophotometry. Chemometric analysis was carried out with PCA and PLS-DA. The maximum wavelengths of chloramphenicol are sequentially from zero to third derivative, 278 nm, 260 nm, 234 nm, 292 nm. The method has good linearity (0.995 < r < 1). Chloramphenicol tends to be degraded to a lesser extent in acids and greater in alkalis and thermals. Chemometric analysis showed acid samples cluster near controls. Derivative spectrophotometry can show the percentage of chloramphenicol degradation although it cannot be certain regarding the degradant. In the chemometric analysis, the degradation was obtained based on the grouping of control and treatment samples.

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