Update Artikelilmiah: 37674

ANISHA SHABILLA

NIM

Judul Artikel

Abstrak (Bhs. Indonesia)

Urease merupakan enzim yang mengkatalisis hidrolisis urea menjadi karbondioksida dan amonia. Urease memiliki banyak manfaat di berbagai bidang sehingga perlu dilakukan eksplorasi terhadap manfaat dari enzim urease. Tujuan dari penelitian ini adalah mengekstraksi enzim urease dari biji papaya (Carica papaya L.), karakterisasi, serta uji aktivitas antibakteri terhadap Staphylococcus aureus. Penelitian ini diawali dengan ekstraksi enzim urease dari biji pepaya sehingga diperoleh ekstrak kasar enzim urease. Karakterisasi enzim urease meliputi, variasi konsentrasi substrat, pH dan suhu inkubasi. Aktivitas urease diuji dengan metode Nessler menggunakan spektrofotometer UV-Vis pada λ 500 nm. Ekstrak kasar enzim urease selanjutnya diuji aktivitas antibakterinya terhadap S. aureus menggunakan metode difusi sumur. Hasil penelitian diperoleh enzim urease dari biji pepaya optimum pada konsentrasi substrat 0,25 M; pH 7; suhu inkubasi 35 ℃ dengan aktivitas sebesar 17,023 U/mL. Laju  maksimum 28,901 U/mL dan konstanta Michaelis-Menten yang diperoleh adalah sebesar 0,179 M. Hasil uji aktivitas antibakteri ekstrak kasar enzim urease dengan konsentrasi 100% diperoleh zona hambat sebesar 3,76 mm.

Abtrak (Bhs. Inggris)

Urease is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease has many benefits in various fields so it is necessary to explore the benefits of the urease enzyme. The purpose of this study was to extract the urease enzyme from papaya seeds (Carica papaya L.), characterize, and test its antibacterial activity against Staphylococcus aureus. This research begins with the extraction of the enzyme urease from papaya seeds in order to obtain a crude extract of the enzyme urease. The characterization of the urease enzyme included variations in substrate concentration, pH and incubation temperature. The urease activity was tested by the Nessler method using a UV-Vis spectrophotometer at 500 nm. The crude extract of the urease enzyme was then tested for its antibacterial activity against S. aureus using the well diffusion method. The results showed that the urease enzyme from papaya seeds was optimum at a substrate concentration of 0.25 M; pH 7; incubation temperature of 35 ℃ with an activity of 17,023 U/mL. The maximum rate of 28.901 U/mL and the Michaelis-Menten constant obtained was 0.179 M/minute. The results of the antibacterial activity of the crude extract of urease enzyme with a concentration of 100% obtained an inhibition zone of 3.76 mm.

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