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VATIMATUZ ZAHRA ANDHALUCYA SUBCHAN
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ISOLATION AND CHARACTERIZATION AEROBIC SULFATE OXIDIZING BACTERIA CAUSING METAL CORROSION
Abstrak (Bhs. Indonesia)
The existence of corrosion is defined as an activity of material destruction which contributes significantly to material degradation in all aspects of the activity. The corrosion rate is highly dependent on many factors such as temperature, pH, and oxygen levels. Sulfate oxidizing bacteria are a class of aerobic bacteria that obtain energy from sulfate oxidation or elemental sulfur to produce sulfates. The colonization of the oxidizer is usually attached to an aerobic environment because a solution with dissolved oxygen will increase the rate of corrosion. The problems studied in this research were whether aerobic oxidizing bacteria causing metal corrosion can be isolated from the corrosive iron, how many population number of aerobic sulfate oxidizing bacteria in the corrosive iron, and what are the identity of the aerobic sulfate oxidizing bacterial isolates involved in the metal corrosion The purposes of the research were to isolate aerobic sulfate oxidizing bacteria involved in the iron corrosive on the ship, to determine the population number of aerobic sulfate oxidizing bacteria, and to identify characteristics of the aerobic sulfate oxidizing bacteria. The research was carried out with survey methods by isolating bacteria from corrosive iron samples using a selective medium of thiosulfate, enumeration of bacterial population was done by a total plate count method, and characterization aerobic sulfate oxidizing bacteria was conducted by a conventional method. The results found 10 isolates (coded as SH1, SH2, SH3, WB1, WB2, WB3, WB4, WB5, FH1, FH2) from three part of the ship such as breaker wave, front hull, and side hull. The population number of aerobic sulfate oxidizing bacteria from corrosive samples on the ship as ranging from 1.59 x 106 – 5.6 x 106 CFU/g. Identification result showed that the 10 isolates belonged to the aerobic sulfate oxidizing bacteria, and the two isolates of WB3 and FH1 were capable of producing sulfate. Those two isolates were identified as species members of the genera Thiobacillus and Sulfobacillus, respectively.
Abtrak (Bhs. Inggris)
The existence of corrosion is defined as an activity of material destruction which contributes significantly to material degradation in all aspects of the activity. The corrosion rate is highly dependent on many factors such as temperature, pH, and oxygen levels. Sulfate oxidizing bacteria are a class of aerobic bacteria that obtain energy from sulfate oxidation or elemental sulfur to produce sulfates. The colonization of the oxidizer is usually attached to an aerobic environment because a solution with dissolved oxygen will increase the rate of corrosion. The problems studied in this research were whether aerobic oxidizing bacteria causing metal corrosion can be isolated from the corrosive iron, how many population number of aerobic sulfate oxidizing bacteria in the corrosive iron, and what are the identity of the aerobic sulfate oxidizing bacterial isolates involved in the metal corrosion The purposes of the research were to isolate aerobic sulfate oxidizing bacteria involved in the iron corrosive on the ship, to determine the population number of aerobic sulfate oxidizing bacteria, and to identify characteristics of the aerobic sulfate oxidizing bacteria. The research was carried out with survey methods by isolating bacteria from corrosive iron samples using a selective medium of thiosulfate, enumeration of bacterial population was done by a total plate count method, and characterization aerobic sulfate oxidizing bacteria was conducted by a conventional method. The results found 10 isolates (coded as SH1, SH2, SH3, WB1, WB2, WB3, WB4, WB5, FH1, FH2) from three part of the ship such as breaker wave, front hull, and side hull. The population number of aerobic sulfate oxidizing bacteria from corrosive samples on the ship as ranging from 1.59 x 106 – 5.6 x 106 CFU/g. Identification result showed that the 10 isolates belonged to the aerobic sulfate oxidizing bacteria, and the two isolates of WB3 and FH1 were capable of producing sulfate. Those two isolates were identified as species members of the genera Thiobacillus and Sulfobacillus, respectively.
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