Home
Login.
Artikelilmiahs
35783
Update
ALIFIA RISA
NIM
Judul Artikel
Biosurfactant Screening and Its Antimicrobial Activity of Lactic Acid Bacteria (LAB) Isolated from Logending Beach Sediment Against Salmonella typhi
Abstrak (Bhs. Indonesia)
Surfaktan yang disintesis secara kimia tidak dapat terurai, mereka tetap beracun bagi lingkungan. Surfaktan turunan mikroba, biosurfaktan (BS) memiliki biodegradabilitas yang lebih tinggi dan menjadikannya alternatif ramah lingkungan. Bertindak sebagai agen anti-perekat, biosurfaktan digunakan untuk mencegah pertumbuhan bakteri patogen bawaan makanan, termasuk Salmonella. Bakteri asam laktat yang diisolasi dari sedimen mangrove di pantai Logending belum diketahui kemampuannya menghasilkan BS dan menghambat Salmonella typhi. Penelitian ini bertujuan untuk menyaring dan menyeleksi isolat BAL dari sedimen pantai Logending yang mampu menghasilkan BS, mengamati daya hambat BS yang dihasilkan isolat BAL terhadap pertumbuhan Salmonella typhi melalui metode antiperekat, dan untuk mengetahui persentase penghambatan Salmonella typhi dan konsentrasi BS terhadap Salmonella typhi. Metode survei digunakan untuk menyeleksi isolat yang mampu menghasilkan biosurfaktan dan zat antiperekat terhadap Salmonella typhi. Variabel bebas dalam penelitian ini adalah isolat BAL penghasil BS, sedangkan variabel terikatnya adalah kemampuan isolat BAL menghasilkan BS dan daya hambat produksi BS terhadap Salmonella typhi. Parameter yang diamati adalah jumlah dan tipikal koloni BAL yang tumbuh pada media BHA, diameter zona bening pada uji hemolitik dan penyebaran minyak, bentuk droplet, dan persentase daya hambat mikroba. Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan dari 12 isolat BAL yang diuji, isolat LG17 mampu menghasilkan biosurfaktan dan memiliki kemampuan sebagai anti-adhesive agent terhadap Salmonella typhi dengan persentase daya hambat mikroba 81,9% untuk konsentrasi BS 0,1 mg/10 mL.
Abtrak (Bhs. Inggris)
Chemically synthesized surfactants are non-biodegradable, they remain toxic to the environment. Microbial-derived surfactant, biosurfactants (BS) has higher biodegradability and make them a green alternative. Acting as an anti-adhesive agent, biosurfactant is used to prevent the growth of food-borne pathogenic bacteria, including Salmonella. Lactic acid bacteria isolated from mangrove sediments at Logending beach is not yet known for its ability to produce BSs and in inhibiting Salmonella typhi. The aims of this research were to screen and select the LAB isolate from Logending beach sediments that able to produce BS, to observe the inhibitory ability of BS produced by LAB isolate against the growth of Salmonella typhi through anti-adhesive method, and to know the percentage of the Salmonella typhi inhibition and concentration of BS against Salmonella typhi. Survey method was used for selecting isolate with the ability to produce biosurfactant and anti-adhesive agent against Salmonella typhi. The independent variable in this research was BSs producer LAB isolates, while dependent variables were the ability of LAB isolates to produce BSs and the inhibition ability of BS produced against Salmonella typhi. The parameters observed were number and typical LAB colony grow in BHA medium, the diameter of clear zone in hemolytic and oil spreading assay, the shape of droplet, and percentage of microbial inhibition. The data obtained were analyzed descriptively. The results showed from 12 LAB isolates tested, LG17 isolate was able to produce biosurfactant and had the ability as an anti-adhesive agent against Salmonella typhi with the percentage of microbial inhibition 81.9 % for BS concentration of 0.1 mg/10 mL.
Kata kunci
Pembimbing 1
Pembimbing 2
Pembimbing 3
Tahun
Jumlah Halaman
Save