Update Artikelilmiah: 35576

SALSA FARIDA

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Abstrak (Bhs. Indonesia)

Tanaman lempuyang gajah (Zingiber zerumbet) dilaporkan berpotensi sebagai antibakteri. Fungi endofit merupakan alternatif penghasil senyawa bioaktif. Namun faktanya sekitar 70% klaster gen penyandi biosintesis tidak aktif dalam kondisi kultur laboratorium standar, sehingga perlu menerapkan strategi OSMAC (One Strain MAny Compounds). Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi fungi endofit dari daun Z. zerumbet dan mengevaluasi aktivitas antibakterinya. Daun Z. zerumbet segar dan sehat dikultur pada media PDA hingga diperoleh isolat fungi murni. Isolat fungi diidentifikasi dengan metode PCR dan difermentasikan pada media kedelai dan beras (kontrol). Selanjutnya diekstraksi dengan EtOAc secara shaking dan dianalisis fitokimia dengan metode KLT dan HPLC. Selain itu, ekstrak fungi endofit diuji aktivitas antibakterinya terhadap Staphylococus aureus dan Escherichia coli dengan metode difusi cakram KirbyBauer. Fungi endofit yang diisolasi dari daun Z. zerumbet teridentifikasi sebagai Meyerozyma carpophila strain H6.3. Ekstrak EtOAc fungi M. carpophila mengandung senyawa terpenoid berdasarkan profil KLT. Berdasarkan profil HPLC, peak ekstrak M. carpophila di media kedelai pada waktu retensi 25,339 menit memiliki spektrum UV yang berbeda. Namun demikian, ekstrak tersebut tidak dapat menghambat pertumbuhan bakteri S. aureus dan E. coli sebab termasuk kategori resisten menurut CLSI (2020). Ekstrak fungi M. carpophila yang diisolasi dari daun lempuyang gajah (Z. zerumbet) dan difermentasikan pada media kedelai tidak menunjukkan aktivitas antibakteri

Abtrak (Bhs. Inggris)

Lempuyang gajah (Zingiber zerumbet) is reported to have antibacterial potential. Endophytic fungi are an alternative way to produce bioactive compounds. However, in fact, about 70% of the biosynthetic coding gene clusters are inactive under standard laboratory culture conditions, so it is necessary to apply the OSMAC (One Strain, Many Compounds) strategy. This study aimed to isolate and identify endophytic fungi from Z. zerumbet leaves and evaluate their antibacterial activity. Fresh and healthy Z. zerumbet leaves were cultured on PDA media to obtain pure fungal isolates. Fungal isolates were identified by the PCR method and fermented on soybean and rice media (control). Furthermore, it was extracted by shaking EtOAc and analyzed for phytochemicals by TLC and HPLC methods. In addition, the endophytic fungi extract was tested for its antibacterial activity against Staphylococus aureus and Escherichia coli by the Kirby-Bauer disk diffusion method. The endophytic fungi isolated from Z. zerumbet leaves were identified as Meyerozyma carpophila strain H6.3. The EtOAc fungus extract of M. carpophila contains terpenoid compounds based on the TLC profile. Based on the HPLC profile, the peak of M. carpophila extract in soybean media at a retention time of 25.339 minutes had a different UV spectra. However, the extract could not inhibit the growth of S. aureus and E. coli bacteria because they were included in the resistant category according to CLSI (2020).  The extract of the fungus M. carpophila, isolated from lempuyang gajah (Z. zerumbet) leaves and fermented in soybean media, did not show antibacterial activity

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