Update Artikelilmiah: 35002

ARY NAHDIYANI AMALIA

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Abstrak (Bhs. Indonesia)

Pengurangan durasi tidur merusak hepar melalui mekanisme stres oksidatif. Purwoceng (Pimpinella pruatjan Molk.) adalah tanaman herbal asli Indonesia yang diketahui memiliki aktivitas antioksidan pada hepar dan potensinya dapat ditingkatkan dalam teknologi nanoemulsi. Tujuan penelitian untuk mengetahui pengaruh nanoemulsi purwoceng terhadap kadar enzim transaminase, volume hepar dan histopatologi hepar tikus putih Wistar jantan pasca induksi stres Paradoxical Sleep Deprivation (PSD) selama 96 jam. Penelitian berupa eksperimental dengan desain post-test only with control group pada 24 ekor tikus yang dibagi menjadi 6 kelompok perlakuan, yaitu kelompok A diberi PSD, kelompok B diberi PSD dan sleep recovery, kelompok C diberi PSD dan ekstrak purwoceng 25 mg/300 gBB/hari, kelompok D diberi PSD dan nanoemulsi purwoceng 25 mg/300 gBB/hari, kelompok E diberi PSD dan nanoemulsi purwoceng 50 mg/300 gBB/hari, serta kelompok F diberi PSD dan nanoemulsi purwoceng 75 mg/300 gBB/hari. Kadar SGPT dan SGOT serum diukur dengan metode UV test. Organ hepar diambil untuk mengukur volume dan dinilai kerusakannya menggunakan skor Manja Roenigk. Data dianalisis dengan uji One Way ANOVA dan dilanjutkan uji Post Hoc Bonferroni. Tidak terdapat perbedaan bermakna pada rerata kadar SGPT (p=0,307), kadar SGOT (p=0,412) dan volume hepar (p=0,227), serta terdapat perbedaan yang bermakna pada rerata skor Manja Roenigk antara kelompok B dibanding kelompok D (p=0,010), kelompok E dan F (p=0,000). Pemberian nanoemulsi purwoceng tidak mempengaruhi enzim transaminase dan volume hepar, tetapi mempengaruhi gambaran histopatologi hepar pasca induksi PSD.

Abtrak (Bhs. Inggris)

Reduction of sleep duration damages the liver through oxidative stress mechanisms. Purwoceng (Pimpinella pruatjan Molk.) is a native Indonesian herbal plant which is known to have antioxidant activity in the liver and its potential can be increased in nanoemulsion technology. The purpose of this study was to determine the effect of purwoceng nanoemulsion on transaminase enzyme levels, liver volume and liver histopathology of male Wistar rats after stress induction of Paradoxical Sleep Deprivation (PSD) for 96 hours. The study was an experimental study with a post-test only design with a control group on 24 rats which were divided into 6 treatment groups, namely group A was given PSD, group B was given PSD and sleep recovery, group C was given PSD and purwoceng extract 25 mg/300 gBB/ day, group D was given PSD and purwoceng nanoemulsion 25 mg/300 gBW/day, group E was given PSD and purwoceng nanoemulsion 50 mg/300 gBW/day, and group F was given PSD and purwoceng nanoemulsion 75 mg/300 gBW/day. Serum SGPT and SGOT levels were measured by the UV test method. Liver organs were taken to measure volume and assessed for damage using the Manja Roenigk score. Data were analyzed by One Way ANOVA test and continued with Bonferroni Post Hoc test. There was no significant difference in the mean SGPT level (p=0.307), SGOT level (p=0.412) and liver volume (p=0.227), and there was a significant difference in the mean score of Manja Roenigk between group B compared to group D (p=0.010), groups E and F (p=0.000). The administration of purwoceng nanoemulsion did not affect the transaminase enzymes and liver volume, but did affect the histopathological picture of the liver after PSD induction.

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