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TYSSA MUTIARA RAMADHANTI KHARIZMA
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EXPLORATION CHITINOLYTIC BACTERIA FROM LOGENDING COASTAL KEBUMEN AND GENUS IDENTIFICATION
Abstrak (Bhs. Indonesia)
Seafood waste in industries may cause environmental problems due to its easy deterioration. However, seafood waste is a significant source of chitin. The enzymatic method can be adopted as an alternative to being more eco-friendly and cost-effective than the chemical method for chitinous degradation. Many groups of chitinolytic bacteria hydrolyze chitin by using chitinase, which benefits the world of biotechnology. This group of bacteria can be found in areas with abundant chitin sources, such as in coastal areas. One of the mangrove environments that has never been studied for chitinolytic bacteria is Logending coastal, Kebumen. This study aimed to select chitinolytic bacteria with the highest chitinolytic activity, identify chitinolytic bacteria morphologically and physiologically, and observe the characteristics of crude chitinase by variation of various pH and temperature. The research methods used were survey method and experimental method. The research stages included subculture, screening chitinolytic bacteria, determination bacteria log phase by observing growth standard and growth curve, conducting semi-quantitative chitinase assay, conducting crude chitinase production, conducting chitinase characterization, and identification bacteria phenetically based on morphological, biochemical, and physiological characters. The survey method was used to select and identify isolates having the highest chitinolytic index by phenetic identification. The experimental method used complete randomized design (CRD) to characterize the optimum activity of crude chitinase by assaying it in a variation of pHs and temperatures. The screening data of chitinolytic isolates were analyzed descriptively. Chitinase assay measured the clear zone with chitinolytic index and optical density (OD) value. Chitinase characterization for pH and temperature was recorded by optical density (OD) value and then analyzed by Kruskal-Wallis test. Identification of isolates was referred to Bergey’s Manual of Systematic Bacteriology. A total of 12 isolates were screened, and one isolate was, namely isolate LG147, had the best chitinolytic activity with a chitinolytic index value of 1.791. Isolate LG147 belongs to the genus Oceanobacillus. On three days incubation period, the highest chitinase production was at 48 hours with an OD value of 0.1245 and chitinolytic index value of 2.39. The crude chitinase performed its optimum activity at pH 8.0 and a temperature of 50oC.
Abtrak (Bhs. Inggris)
Seafood waste in industries may cause environmental problems due to its easy deterioration. However, seafood waste is a significant source of chitin. The enzymatic method can be adopted as an alternative to being more eco-friendly and cost-effective than the chemical method for chitinous degradation. Many groups of chitinolytic bacteria hydrolyze chitin by using chitinase, which benefits the world of biotechnology. This group of bacteria can be found in areas with abundant chitin sources, such as in coastal areas. One of the mangrove environments that has never been studied for chitinolytic bacteria is Logending coastal, Kebumen. This study aimed to select chitinolytic bacteria with the highest chitinolytic activity, identify chitinolytic bacteria morphologically and physiologically, and observe the characteristics of crude chitinase by variation of various pH and temperature. The research methods used were survey method and experimental method. The research stages included subculture, screening chitinolytic bacteria, determination bacteria log phase by observing growth standard and growth curve, conducting semi-quantitative chitinase assay, conducting crude chitinase production, conducting chitinase characterization, and identification bacteria phenetically based on morphological, biochemical, and physiological characters. The survey method was used to select and identify isolates having the highest chitinolytic index by phenetic identification. The experimental method used complete randomized design (CRD) to characterize the optimum activity of crude chitinase by assaying it in a variation of pHs and temperatures. The screening data of chitinolytic isolates were analyzed descriptively. Chitinase assay measured the clear zone with chitinolytic index and optical density (OD) value. Chitinase characterization for pH and temperature was recorded by optical density (OD) value and then analyzed by Kruskal-Wallis test. Identification of isolates was referred to Bergey’s Manual of Systematic Bacteriology. A total of 12 isolates were screened, and one isolate was, namely isolate LG147, had the best chitinolytic activity with a chitinolytic index value of 1.791. Isolate LG147 belongs to the genus Oceanobacillus. On three days incubation period, the highest chitinase production was at 48 hours with an OD value of 0.1245 and chitinolytic index value of 2.39. The crude chitinase performed its optimum activity at pH 8.0 and a temperature of 50oC.
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