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AGUNG PRASETYO

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Abstrak (Bhs. Indonesia)

Penelitian ini bertujuan:  1) Melakukan eksplorasi PGPR dari rizosfer nanas, 2) Memperoleh isolat murni PGPR dari rizosfer nanas, 3)Mengetahui karakteristik PGPR isolat murni. Penelitian dilaksanakan pada bulan Februari sampai bulan Oktober 2021 di Desa Pengalusan dan Desa campakoah, Kecamatan Mrebet, Kabupaten Purbalingga, Jawa Tengah dan di Laboratorium agroekologi, Fakultas Pertanian, Universitas jenderal Soedirman, Purwokerto. Penelitian dilaksanakan secara eksperimental,  sampel tanah diambil dari dua tempat dengan ketinggian yang berbeda, yaitu di Desa Pengalusan dan Campakoah. Pengambilan sampel tanah dilakukan secara komposit dengan metode purpossive sampling.  Setelah diperoleh sampel tanah, lalu dilanjutkan dengan isolasi bakteri di medium Nutrient Agar (NA). Kemudian isolat yang telah diperoleh diuji morfologi makroskopis, morfologi mikroskopis, dan uji aktivitas PGPR sebagai variabel pengamatan. Hasil penelitian menunjukkan bahwa populasi bakteri yang berasal dari Desa Campakoah lebih tinggi (15,22 x 106 cfu/ml) dibandingkan populasi bakteri dari sampel tanah Desa Pengalusan (2,68 x 106 cfu/ml).  Dari Desa Pengalusan dan campakoah berhasil diperoleh masing-masing sebanyak 5 isolat. Berdasarkan hasil pengujian, seluruh isolat tergolong bakteri gram positif. Seluruh isolat selain C51 mempunyai kemampuan menghasilkan Indole-3-acetic Acid. Isolat yang mempunyai kemampuan melarutkan Fosfat hanya isolat P61. Isolat yang mempunyai kemampuan menghasilkan enzim protease (proteolitik) adalah isolat C51, C52, C61, C62, P41, dan P61. Seluruh isolat mempunyai kemampuan memfiksasi Nitrogen. Berdasarkan data hasil pengujian, isolat yang mempunyai keempat kemampuan PGPR adalah P61.

Abtrak (Bhs. Inggris)

This research was aimed to 1) Doing exploration of PGPR from pinneaple rizosphere, 2) Obtaining pure isolates from pinneaple rizosphere, 3) Finding out the characteristics the  pure of  isolates. This research was implemented from February until October 2021 at Pengalusan and campakoah Villages, Mrebet District, Purbalingga Regency, Central Java and Laboratory of Agroecology, Faculty of Agriculture, University of Jenderal Soedirman, Purwokerto. This research was implemented experimentally, the samples of soil was took from two different  places with different elevation, those were Pengalusan and campakoah Villages. The taking of soil samples was implemented compositely by purpossive sampling method. After the sample of soils was took, then continued by bactery isolation in Natrium Agar medium. Isolates that has gotten then tested about macroscopic and microscopic morphologies, and the activity of PGPR. The results of research showed that the bacterial population from Campakoah Village higher (15,22 x 106 cfu/ml) than Pengalusan Village (2,68 x 106 cfu/ml). Each from Pengalusan Village and Campakoah Village was succed to collect 5 isolates. Based on the results of test, all of the isolates including in gram possitive bacteria. All of the isolates except C51 have capability to produce Indole-3-acetic Acid. The isolate that have capability to dissolve phospate was only isolate P61. The isolates that have capability to produce protease enzym (proteolytic) were isolate C51, C52, C61, C62, P41, and P61. All of the isolates have capability to fix Nitrogen. The isolate that have completely four capabilities are P61.

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