Update Artikelilmiah: 33004

AJENG PUTRI RETNO ANDANI

NIM

Judul Artikel

Abstrak (Bhs. Indonesia)

Penelitian ini bertujuan untuk mengetahui kemampuan Streptomyces spp. asal sedimen mangrove dalam menghambat pertumbuhan bakteri Vibrio cholerae, mengetahui kekuatan senyawa antibakteri yang dihasilkan oleh Streptomyces spp. terhadap V. cholerae, dan mengetahui karakteristik dari senyawa antibakteri yang dihasilkan oleh Streptomyces spp. Tahap penelitian meliputi penapisan isolat bakteri Streptomyces spp. (SA34, SA37, SA40, SAE4034) yang menghambat V. cholerae, produksi senyawa antibakteri, uji aktivitas antibakteri dengan metode Kirby Bauer, penentuan nilai MIC (%), pemisahan senyawa antibakteri dengan metode TLC, uji fitokimia, uji bioautografi, uji aktivitas senyawa antibakteri pada variasi suhu (40oC, 60oC. 80oC, 100oC) dan pH (2, 4, 6, 9), serta uji konfirmasi karakterisasi bakteri. Data dianalisis secara deskriptif. Hasil penapisan menunjukkan bahwa penghambatan terhadap pertumbuhan V. cholerae tertinggi ditunjukkan oleh isolat SAE4034. Ekstrak kasar mampu menghambat V. cholerae dengan diameter zona hambat 11,5-17,5 mm; nilai MIC 30%, dan menghasilkan senyawa bioaktif dengan nilai Rf berkisar antara 0,31-0,70. Senyawa yang mampu menghambat memiliki nilai Rf 0,43 dan senyawa tersebut termasuk golongan alkaloid. Perlakuan suhu 40oC tetap tinggi dengan zona hambat 14 mm dan aktivitas penghambatannya menurun sejalan dengan perlakuan suhu yang lebih tinggi sampai 100oC. Perlakuan pH 6 menunjukkan  aktivitas penghambatan tertinggi dengan zona hambat 16 mm, sebaliknya pada pH 2, 4, dan 9 aktivitas antibakteri ekstrak kasar menurun.

Abtrak (Bhs. Inggris)

This study aimed to determine the ability of Streptomyces spp. origin of mangrove sediments in inhibiting the growth of Vibrio cholerae bacteria, to know the inhibitory activity of antibacterial compounds produced by Streptomyces spp. against V. cholerae, and to know the characteristics of antibacterial compounds produced by Streptomyces spp.The research stages included the screening of Streptomyces spp. isolates (SA34, SA37, SA40, SAE4034) against V. cholerae, production of antibacterial compounds, test of antibacterial inhibitory activity using Kirby Bauer method, determination of MIC value (%), separation of antibacterial compounds by TLC and phytochemical methods, bioautography test, treatment of temperature variations (40oC, 60oC. 80oC, 100oC) and pH variations (2, 4, 6, 9) over antibacterial compound activity, and confirmation tests of bacterial characteristics. Data were analyzed descriptively. The screening results showed that the highest inhibition of the growth of V. cholerae was shown by Streptomyces sp. SAE4034. The crude extract was able to inhibit V. cholerae with an inhibitory zone diameter of 11.5-17.5 mm, MIC value of 30%, and produced compounds with Rf values ranging from 0.31-0.70. Antibacterial compounds of Streptomyces sp. SAE4034 which was able to inhibit the growth of V. cholerae bacteria had a Rf value of 0.43 and the compound belonged to the alkaloid group. Treated with temperature of 40oC remained high with an inhibition zone of 14 mm and its inhibitory activity decreased with higher temperature treatment until 100oC. Likewise with the pH treatment, the highest inhibitory activity was obtained at pH 6 with an inhibition zone of 16 mm, on the contrary, at pH 2, 4, and 9 the antibacterial activity of the extract decreased.

Kata kunci

Pembimbing 1

Pembimbing 2

Pembimbing 3

Tahun

Jumlah Halaman